Ginsenoside Rb1

Catalog No.S3924 Batch:S392404

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Technical Data

Formula

C54H92O23

Molecular Weight 1109.29 CAS No. 41753-43-9
Solubility (25°C)* In vitro DMSO 100 mg/mL (90.14 mM)
Water 100 mg/mL (90.14 mM)
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Ginsenoside Rb1 (Gypenoside Ⅲ) is a protopanaxadiol that has diverse in vitro and in vivo effects, including neuroprotective, anti-inflammatory, and anti-obesity actions. Ginsenoside Rb1, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with IC50 of 6.3±1.0 μM. Ginsenoside also inhibits IRAK-1 activation and phosphorylation of NF-κB p65. Ginsenoside Rb1 reduces the expressions of TLR3, TLR4 and TRAF-6, and down-regulates the levels of TNF-α, IFN-β and iNOS.
Targets
IRAK-1 [4] NF-κB [4] TLR3 [5] TLR4 [5] TNF-α [5] View More
In vitro

Ginsenoside Rb1 promotes the expressions of Nestin, NSE, GFAP and stimulates the differentiation of NSCs[1]. GRb1 is found to not only inhibit Aβ-induced ROROS overproduction and lipid peroxidation, but also to increase the Bcl-2/Bax ratio and attenuate caspase-3 activation, thereby improving cell survival. GRb1 may therefore act as a ROS scavenger, and such antioxidant properties may play a protective role against Aβ-induced cell injury[2].

In vivo

Ginsenoside Rb1 can increase the ability of spatial learning and neural regeneration. It has an anti-neuroinflammatory effect in a rat model of AD. Oral ginsenoside Rb1 can metabolize ginsenoside compound K through intestinal bacteria, and then play a pharmacological role together with esterification of fatty acids to prevent memory loss and improve spatial learning. Ginsenoside Rb1 increases synaptic density and the expression of brain-derived neurotrophic factors Bcl-2 and antioxidant enzyme in hippocampus of mice, and inhibits apoptosis and calcium overload. Ginsenoside Rb1 can stimulate the release of acetylcholine and enhance the expression of choline acetyltransferase mRNA in rat brain, which contributes to the increase of hippocampal neurogenesis. It significantly increased the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus[1].

Protocol (from reference)

Cell Assay:

[2]

  • Cell lines

    PC12 cells

  • Concentrations

    0.01 mM, 0.1 mM, 1 mM

  • Incubation Time

    24 h

  • Method

    PC12 cells are grown in RPMI-1640 containing 10% heat-inactivated horse serum and 5% fetal bovine serum at 37˚C in a 5% CO2 atmosphere. The medium is changed every other day and cells are plated at the appropriate density according to each experimental scale. To study the effects of GRb1 on Aβ25-35-induced cell injury, cells are pre-incubated with GRb1 for 24 h, and then Aβ25-35 is added to the medium for an additional 24 h. The experimental groups are: A, control; B, 25 μM Aβ25-35; C, 0.01 mM GRb1 + 25 μM Aβ25-35; D, 0.1 mM GRb1 + 25 μM Aβ25-35; E, 1 mM GRb1 + 25 μM Aβ25-35. Following pre-treatment with GRb1 and treatment with Aβ, the cells are subjected to the MTT assay and flow cytometry analysis, the measurement of ROROS and MDADA, Western blotting and immunocytochemistry.

Animal Study:

[1]

  • Animal Models

    Sprague Dawley (SD) male rats

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Selleck's Ginsenoside Rb1 has been cited by 4 publications

Activation of nemo-like kinase in diamond blackfan anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis [ J Biol Chem, 2024, 300(8):107542] PubMed: 38992436
Xuesaitong exerts long-term neuroprotection for stroke recovery by inhibiting the ROCKII pathway, in vitro and in vivo [ J Ethnopharmacol, 2021, S0378-8741(21)00169-0] PubMed: 33617967
The active component of Ginseng, Ginsenoside Rb1, improves erythropoiesis in models of Diamond Blackfan Anemia by targeting Nemo-like Kinase [ J Biol Chem, 2021, S0021-9258(21)00790-0] PubMed: 34298020
Host defense against Neospora caninum infection via IL-12p40 production through TLR2/TLR3-AKT-ERK signaling pathway in C57BL/6 mice [ Mol Immunol, 2021, 139:140-152] PubMed: 34509754

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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