GC7 Sulfate

Catalog No.S2961 Batch:S296102

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Technical Data

Formula

C8H22N4O4S
Molecular Weight 270.35 CAS No. 150417-90-6
Solubility (25°C)* In vitro Water 5 mg/mL (18.49 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description GC7 (N1-guanyl-1,7-diaminoheptane) Sulfate is an inhibitor of deoxyhypusine synthase (DHPS). GC7 inhibits Neuroblastoma (NB) cell proliferation in a dose-dependent manner, through induction of the cell cycle inhibitor p21 and reduction of total and phosphorylated Rb proteins.
Targets
DHPS [1] p21 [1]
In vitro

GC7 Sulfate, a deoxyhypusine synthase (DHPS) inhibitor, inhibits MYCN-amplified and chemotherapy-resistant NB cell proliferation, regulates several cell cycle controlling proteins and triggers arrest via the p21/Cdk4/Rb signaling axis, even in the presence of MYCN.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    MYCN2 (±Dox) and BE(2)-C cells

  • Concentrations

    0.1-100 μM

  • Incubation Time

    72 h

  • Method

    Cell viability was analyzed with the MTS assay kit. Briefly, after treatment with GC7 inhibitor for 72 h in 96-well plates, cells were directly incubated with the MTS dye reagent for 1 h at 37 ℃ in a 5 % CO2 atmosphere.

Selleck's GC7 Sulfate has been cited by 1 publication

Glutamine-derived aspartate is required for eIF5A hypusination-mediated translation of HIF-1α to induce the polarization of tumor-associated macrophages [ Exp Mol Med, 2024, 10.1038/s12276-024-01214-1] PubMed: 38689086

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.