Gboxin

Catalog No.S8828 Batch:S882801

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Technical Data

Formula

C22H33ClN2O2
Molecular Weight 392.96 CAS No. 2101315-36-8
Solubility (25°C)* In vitro Ethanol 79 mg/mL (201.03 mM)
DMSO 39 mg/mL (99.24 mM)
Water 2 mg/mL (5.08 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.97mg/ml Taking the 1 mL working solution as an example, add 50 μL of 19.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.95mg/ml Taking the 1 mL working solution as an example, add 50 μL of 39 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Gboxin is an inhibitor of oxidative phosphorylation in cancer cells. Gboxin inhibits the activity of F0F1 ATP synthase. It specifically inhibits the growth of primary mouse and human glioblastoma cells but not that of mouse embryonic fibroblasts or neonatal astrocytes.
Targets
oxidative phosphorylation [1] F0F1 ATP synthase [1]
In vitro

Gboxin rapidly and irreversibly compromises GBM oxygen consumption. Reliant on its positive charge, Gboxin associates with mitochondrial oxidative phosphorylation complexes in a proton gradient dependent manner and inhibits F0F1 ATP synthase activity. Gboxin targets unique features of mitochondrial pH in GBM and other cancer cells, independent of their genetic composition, and exerts its tumor cell specific toxicity in primary culture and in vivo[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HTS, MEF and astrocyte cells

  • Concentrations

    0-15 μM

  • Incubation Time

    96 h

  • Method

    --

Selleck's Gboxin has been cited by 4 publications

Multivariate analysis of metabolic state vulnerabilities across diverse cancer contexts reveals synthetically lethal associations [ Cell Rep, 2024, 43(10):114775] PubMed: 39305483
Targeting mitochondrial energetics reverses panobinostat- and marizomib-induced resistance in pediatric and adult high-grade gliomas [ Mol Oncol, 2023, 17(9):1821-1843] PubMed: 37014128
Targeting mitochondrial energetics reverses panobinostat- and marizomib-induced resistance in pediatric and adult high-grade gliomas [ Mol Oncol, 2023, 17(9):1821-1843] PubMed: 37014128
Disrupting metformin adaptation of liver cancer cells by targeting the TOMM34/ATP5B axis [ EMBO Mol Med, 2022, 14(12):e16082] PubMed: 36321555

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.