Garlic oil

Catalog No.E0651 Batch:E065101

Technical Data

Formula	N/A
Molecular Weight		CAS No.	8000-78-0
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description	Garlic oil blocks tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis by inducing phase II drug-metabolizing enzymes.
In vitro	Garlic oil exhibits favorable anti‑cancer potential towards gliomas of various degrees of differentiation. Garlic oil induces apoptotic death in Human astrocytoma cell lines at a low concentration(0.75 µg/ml-1.5 µg/ml). ^[2]
In vivo	Garlic oil supplementation during pregnancy and lactation protects against hypertension induced by high-fat (HF) diet in adult male offspring. The beneficial effects of garlic oil are associated with increased renal mRNA expression and activity of Hydrogen sulfide (H2 S)-generating enzymes, increased NO bioavailability, increased plasma short chain fatty acid levels, and alterations of gut microbiota composition. Garlic oil supplementation increases abundance of genus Lactobacillus, but decreases genera Turicibacter and Staphylococcus.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Human astrocytoma cell lines Concentrations 0.015 µg/ml-150 µg/ml Incubation Time 24 h Method Human astrocytoma cell lines are incubated with 15 or 150 µg/ml garlic oil for 24 h, and then used for the MTT assay to determine viability of cells. Annexin V conjugated to fluorescein isothiocyanate (FITC) fluorochrome is used for flow cytometry analysis of cells which are undergoing apoptosis.
Animal Study:^{^[3]}	Animal Models Pregnant rats Dosages 100 mg/kg Administration o.g.

Cell Assay:^{^[2]}

Cell lines
Human astrocytoma cell lines
Concentrations
0.015 µg/ml-150 µg/ml
Incubation Time
24 h
Method
Human astrocytoma cell lines are incubated with 15 or 150 µg/ml garlic oil for 24 h, and then used for the MTT assay to determine viability of cells. Annexin V conjugated to fluorescein isothiocyanate (FITC) fluorochrome is used for flow cytometry analysis of cells which are undergoing apoptosis.

Animal Study:^{^[3]}

Animal Models
Pregnant rats
Dosages
100 mg/kg
Administration
o.g.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.