Galloflavin

Catalog No.S9606 Batch:S960601

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Technical Data

Formula

C12H6O8
Molecular Weight 278.17 CAS No. 568-80-9
Solubility (25°C)* In vitro DMSO 2 mg/mL (7.18 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Galloflavin (NSC 107022) is a novel inhibitor of lactate dehydrogenasee (LDH) with Ki values of 5.46 μM and 15.06 μM for LDH-A and LDH-B, respectively.
Targets
LDH-A [1]
(Cell-free assay)		 LDH-B [1]
(Cell-free assay)
5.46 µM(Ki) 15.06 µM(Ki)
In vitro

Galloflavin inhibits both human LDH isoforms by preferentially binding the free enzyme, without competing with the substrate or cofactor. The calculated Ki values for pyruvate are 5.46 mm (LDH-A) and 15.06 mm (LDH-B). In cultured tumor cells, galloflavin blocks aerobic glycolysis at micromolar concentrations,  not interferes with cell respiration, and induces cell death by triggering apoptosis.[1]
In vivo

Galloflavin treatment attenuates the malignant and invasion abilities of colon cancer cells in tumor-bearing mice, which are inhibited by the knockout of NLRP3.[2]

Protocol (from reference)

Kinase Assay:

[1]
  • LDH activity assay

    Human LDH-A (from human liver) and LDH-B (from human heart) are added in scalar amounts (0– 500 mm final concentrations) to a reaction mix containing 100 mm phosphate buffer (pH 7.5), LDH (0.015 UmL-1), 1 mm pyruvate, and 150 mm NADH. The enzymatic activity is measured by monitoring NADH oxidation for a period of 3 min (lex= 340 nm, lem =460 nm).
Cell Assay:

[1]
  • Cell lines

    PLC/PRF/5 cell line

  • Concentrations

    0.1–500 μM

  • Incubation Time

    3 h

  • Method

    PLC/PRF/5 cells (1.6 × 104 per well) are seeded in 24-well plates, allowed to adhere overnight, and treated for 72 h at 37 ℃ with  0–500 μM dose of Galloflavin. After the treatments, cells are used for assay of lactic acid production, cellular respiration and cell proliferation.

Selleck's Galloflavin has been cited by 1 publication

Lactate-lactylation-HSPA6 axis promotes PRRSV replication by impairing IFN-β production [ J Virol, 2024, 98(1):e0167023] PubMed: 38088561

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.