Gallic acid

Catalog No.S4603 Batch:S460302

Technical Data

Formula

C₇H₆O₅

Molecular Weight

170.12

CAS No.

149-91-7

Solubility (25°C)*

In vitro

DMSO

34 mg/mL (199.85 mM)

Ethanol

34 mg/mL (199.85 mM)

Water

9 mg/mL (52.9 mM)

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.7mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.34mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 6.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Gallic acid (3,4,5-Trihydroxybenzoic acid, Gallate, Benzoic acid), known as powerful antioxidants, is an organic acid found in a variety of foods and herbs.
In vitro	Gallic acid does not protect against H(2)O(2)-induced PC12 cell death. It reduced the viability of PC12 cells in a dose-dependent manner. Gallic acid also induces cleavage of poly (ADP-ribose) polymerase, which is strongly related to apoptosis in neurons. Gallic acid induces the phosphorylation of c-Jun N-terminal protein kinase (JNK) and the downregulation of Bcl-2 in PC12 cells. Gallic acid leads to a progressive reduction in the viability of vector-transfected PC12 cells, which is delayed in PC12 cells that overexpressed Bcl-2^[2].
In vivo	Gallic acid effectively protects rat erythrocytes. The antioxidant effect of the compound at the tested dosage in vivo was more prevalent than its prooxidative effects. Gallic acid has ameliorative effect on lipid peroxidation in vivo^[1].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines PC12 cells Concentrations 25 or 50 μM Incubation Time 24 h Method Cell viability is measured using the MTT assay. PC12 cells are incubated at 37◦C with H2O2 or gallic acid at different concentrations for 24 h with or without pretreatment with resveratrol. They are then treated with the MTT solution (final concentration, 1 mg/mL) for 2 h. The dark-blue formazan crystals that formed in intact cells are dissolved in DMSO, and the absorbance at 570 nm is measured using a micro-ELISA reader.
Animal Study:^{^[1]}	Animal Models Male Wistar rats Dosages 10 mg/kg and 20 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
PC12 cells
Concentrations
25 or 50 μM
Incubation Time
24 h
Method
Cell viability is measured using the MTT assay. PC12 cells are incubated at 37◦C with H2O2 or gallic acid at different concentrations for 24 h with or without pretreatment with resveratrol. They are then treated with the MTT solution (final concentration, 1 mg/mL) for 2 h. The dark-blue formazan crystals that formed in intact cells are dissolved in DMSO, and the absorbance at 570 nm is measured using a micro-ELISA reader.

Animal Study:^{^[1]}

Animal Models
Male Wistar rats
Dosages
10 mg/kg and 20 mg/kg
Administration
i.p.

References

Selleck's Gallic acid has been cited by 5 publications

Antioxidant effect of gallic acid on retinal ganglion cells in glaucoma model [ Sci Rep, 2024, 14(1):14907]	PubMed: 38942959
Gallic acid pretreatment mitigates parathyroid ischemia-reperfusion injury through signaling pathway modulation [ Sci Rep, 2024, 14(1):12971]	PubMed: 38839854
Single-cell profiling reveals a potent role of quercetin in promoting hair regeneration [ Protein Cell, 2023, 14(6):398-415]	PubMed: 37285263
IDH2 stabilizes HIF-1α-induced metabolic reprogramming and promotes chemoresistance in urothelial cancer [ EMBO J, 2023, e110620.]	PubMed: 36637036
Resveratrol compounds inhibit human holocarboxylase synthetase and cause a lean phenotype in Drosophila melanogaster. [ J Nutr Biochem, 2015, 26(11):1379-84]	PubMed: 26303405

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.