G150

Catalog No.S8944 Batch:S894402

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Technical Data

Formula

C18H16Cl2N4O2
Molecular Weight 391.25 CAS No. 2369751-30-2
Solubility (25°C)* In vitro DMSO 78 mg/mL (199.36 mM)
Ethanol 1.5 mg/mL (3.83 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
1.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description G150 is a potent and highly selective human cyclic GMP-AMP synthase (cGAS) inhibitor with IC50 of 10.2 nM.
Targets
h-cGAS [1]
(Cell-free assay)
10.2 nM
In vitro

The most potent h-cGAS-specific derivative with 2-amino pyridine (G150) shows inhibitory activity in THP1 cells and primary human macrophages while a complete absence of off-target effect on a diverse range of sensors. G150 also shows no substantial cellular toxicity[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    THP1 cells, primary macrophage cells

  • Concentrations

    10 μM and 5 μM

  • Incubation Time

    1 h

  • Method

    Cellular activation of cGAS enzyme leads to IFNB1 mRNA expression in THP1, and primary human macrophage cells and is quantified using qRT-PCR. Total RNA is isolated from 5 × 105 THP1 cells per well of a 12-well plate, which are pre-incubated with G150 for 1 h. Human primary macrophages are used at 3×105 cells per well of a 12-well plate.

Selleck's G150 has been cited by 4 publications

Polystyrene microplastics induce pulmonary fibrosis by promoting alveolar epithelial cell ferroptosis through cGAS/STING signaling [ Ecotoxicol Environ Saf, 2024, 277:116357] PubMed: 38677073
Interleukin-1β promotes human metapneumovirus replication via activating the cGAS-STING pathway [ Virus Res, 2024, 343:199344] PubMed: 38431054
A novel TREX1 inhibitor, VB-85680, upregulates cellular interferon responses [ PLoS One, 2024, 19(8):e0305962] PubMed: 39178223
HIV-1-induced type I IFNs promote viral latency in macrophages [ J Leukoc Biol, 2022, 10.1002/JLB.4MA0422-616R] PubMed: 35588262

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Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.