Furazolidone

Catalog No.S4528 Batch:S452801

Technical Data

Formula	C₈H₇N₃O₅
Molecular Weight	225.16	CAS No.	67-45-8
Solubility (25°C)*	In vitro	DMSO	45 mg/mL (199.85 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Furazolidone(Furoxone) is a synthetic nitrofuran derivative with bactericidal or bacteriostatic activity when used against Gram-positive and Gram-negative bacteria.
In vitro	Furazolidone displays potent antiproliferative properties at submicromolar concentrations and induces apoptosis in AML cell lines. Furazolidone treatment of certain AML cells induces myeloid cell differentiation by morphology and flow cytometry for CD11b expression, resulting in increased stability of tumor suppressor p53 protein in AML cells^[1].
In vivo	FZ accelerates its own metabolism in the chicken by induction of the activity of CPR whereas no effect is observed in the rat^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines The leukemic cells(Kasumi-1, NB4 and MolM-13) Concentrations from 1 µM to 50 µM Incubation Time 24, 48 and 72 hours Method Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×10⁴ viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of FZD ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with FZD for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate.
Animal Study:^{^[2]}	Animal Models Wistar rats and White Leghorn chickens Dosages 62.5 mg/kg/day; 125 mg/kg/day Administration orally by crop tube

Cell Assay:^{^[1]}

Cell lines
The leukemic cells(Kasumi-1, NB4 and MolM-13)
Concentrations
from 1 µM to 50 µM
Incubation Time
24, 48 and 72 hours
Method
Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×10⁴ viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of FZD ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with FZD for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate.

Animal Study:^{^[2]}

Animal Models
Wistar rats and White Leghorn chickens
Dosages
62.5 mg/kg/day; 125 mg/kg/day
Administration
orally by crop tube

References

Selleck's Furazolidone has been cited by 1 publication

The anti-infection drug furazolidone inhibits NF-κB signaling and induces cell apoptosis in small cell lung cancer [ Kaohsiung J Med Sci, 2020, 10.1002/kjm2.12281]	PubMed: 32767507

The anti-infection drug furazolidone inhibits NF-κB signaling and induces cell apoptosis in small cell lung cancer [ Kaohsiung J Med Sci, 2020, 10.1002/kjm2.12281]

PubMed: 32767507

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.