Forskolin (Colforsin)

Catalog No.S2449 Batch:S244907

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Technical Data

Formula

C22H34O7

Molecular Weight 410.5 CAS No. 66575-29-9
Solubility (25°C)* In vitro DMSO 40 mg/mL (97.44 mM)
Ethanol 20 mg/mL (48.72 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Forskolin (Colforsin) is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP in the study and research of cell physiology. Forskolin also activates PXR and FXR activity. Forskolin stimulates autophagy.
Targets
Adenylyl cyclase (AC) [1]
(A wide variety of cell types)
In vitro

Forskolin increases cAMP levels in preparations of membranes, cells, or tissues. Forskolin not only activates AC but also interacts with certain other proteins, including glucose transporters and ion channels. Forskolin is able to promote activation of nine different transmembrane iso-forms of AC, albeit with somewhat less efficacy for AC9, which could be used to provides a means to identify and quantify high-affinity binding sites, i.e., G-proteins (Gs)–AC complexes. Activation of s by GPCRs contributes to Forskolin-stimulated cAMP generation in cells because of s-Forskolin potentiation of AC activity. [1] Forskolin stimulates adenylate cyclase activity without interacting with cell surface receptors. Forskolin's potentiation of cAMP in turn inhibits basophil and mast cell degranulation and histamine release, lowers blood pressure and intraocular pressure, inhibits platelet aggregation, promotes vasodilation, bronchodilation, and thyroid hormone secretion, and stimulates lipolysis in fat cells. Forskolin inhibits the binding of platelet-activating factor (PAF), independently of cAMP formation, which may be a result of Forskolin's direct effect on PAF or via interference with PAF binding to receptor sites. Forskolin also appears to have an effect on several membrane transport proteins, and inhibits glucose transport in erythrocytes, adipocytes, platelets, and other cells. Forskolin is used to treat with glaucoma. [2]

In vivo

Colforsin (Forskolin, HL 362, Coleonol) is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types, commonly used to raise levels of cAMP. It also activates PXR and FXR activity and stimulates autophagy.[4]

Protocol (from reference)

Cell Assay:

[3]

  • Cell lines

    hMPCs or cynMPCs

  • Concentrations

    20 μM

  • Incubation Time

    2 h

  • Method

    Cells were treated with 20 μM forskolin (S2449, Selleck) for 2 h for synchronization.

Animal Study:

[4]

  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    10 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Eur J Cancer, 2014, 50(7), 1310-20]

Data from [Data independently produced by , , Clin Cancer Res, 2018, doi:10.1158/1078-0432.CCR-18-2912]

Data from [Data independently produced by , , J Exp Clin Cancer Res, 2019, 37(1):288]

Data from [Data independently produced by , , Mol Cancer Res, 2017, 15(12):1792-1802]

Selleck's Forskolin (Colforsin) has been cited by 158 publications

Directed Differentiation of Human Induced Pluripotent Stem Cells to Heart Valve Cells [ Circulation, 2024, 149(18):1435-1456] PubMed: 38357822
Preclinical efficacy and safety of encapsulated proliferating human hepatocyte organoids in treating liver failure [ Cell Stem Cell, 2024, S1934-5909(24)00048-1] PubMed: 38458193
Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 [ iScience, 2024, 27(2):108857] PubMed: 38303710
IL-6-Derived Autocrine Lactate Promotes Immune Escape of Uveal Melanoma [ Invest Ophthalmol Vis Sci, 2024, 65(3):37] PubMed: 38551584
IL-6-Driven Autocrine Lactate Promotes Immune Escape of Uveal Melanoma [ Invest Ophthalmol Vis Sci, 2024, 65(3):37] PubMed: 38551584
Generation of marmoset primordial germ cell-like cells under chemically defined conditions [ Life Sci Alliance, 2024, 7(6)e202302371] PubMed: 38499329
Sox9 links biliary maturation to branching morphogenesis [ bioRxiv, 2024, 2024.01.15.574730] PubMed: 38293117
A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals [ Cell Stem Cell, 2023, 30(7):938-949.e7] PubMed: 37343565
A placental model of SARS-CoV-2 infection reveals ACE2-dependent susceptibility and differentiation impairment in syncytiotrophoblasts [ Nat Cell Biol, 2023, 25(8):1223-1234] PubMed: 37443288
Kinase-Modulated Bioluminescent Indicators Enable Noninvasive Imaging of Drug Activity in the Brain [ ACS Cent Sci, 2023, 9(4):719-732] PubMed: 37122464

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.