|
Toll Free: (877) 796-6397 -- USA and Canada only -- |
Fax: +1-832-582-8590 Orders: +1-832-582-8158 |
Tech Support: +1-832-582-8158 Ext:3 Please provide your Order Number in the email. |
Technical Data
| Formula | C25H30N2O5 |
|||
| Molecular Weight | 438.52 | CAS No. | 313967-18-9 | |
| Solubility (25°C)* | In vitro | DMSO | 88 mg/mL (200.67 mM) | |
| Ethanol | 8 mg/mL (18.24 mM) | |||
| Water | Insoluble | |||
|
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
||||
Preparing Stock Solutions
Biological Activity
| Description | FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM. | ||
|---|---|---|---|
| Targets |
|
||
| In vitro | In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. FLI-06 changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. FLI-06 disrupt the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER. [1] | ||
| In vivo | FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos. [1] |
Protocol (from reference)
| Animal Study: |
|
|---|
Customer Product Validation
-
Data from [Data independently produced by , , Am J Cancer Res, 2017, 7(9):1804-1819]
-
Data from [Data independently produced by , , Biochem Biophys Res Commun, 2016]
Selleck's FLI-06 has been cited by 12 publications
| Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells [ Dev Cell, 2022, 57(2):260-276.e9] | PubMed: 35077680 |
| Autophagy-based-unconventional-secretion-of-HMGB1-by-keratinocytes-plays-a-pivotal-role-in-psoriatic-skin-inflammation. [ Autophagy, 2020, 16:1-24] | PubMed: 32019420 |
| NCSTN promotes hepatocellular carcinoma cell growth and metastasis via β-catenin activation in a Notch1/AKT dependent manner [ J Exp Clin Cancer Res, 2020, 39(1):128] | PubMed: 32631394 |
| HDAC Inhibitor LBH589 Suppresses the Proliferation but Enhances the Antileukemic Effect of Human γδT Cells [ Mol Ther Oncolytics, 2020, 18:623-630] | PubMed: 33005729 |
| MYCN-mediated regulation of the HES1 promoter enhances the chemoresistance of small-cell lung cancer by modulating apoptosis. [ Am J Cancer Res, 2019, 9(9):1938-1956] | PubMed: 31598396 |
| FLI-06 Intercepts Notch Signaling And Suppresses The Proliferation And Self-renewal Of Tongue Cancer Cells. [ Onco Targets Ther, 2019, 12:7663-7674] | PubMed: 31571917 |
| Astrocyte Elevated Gene 1 (AEG-1) Acts as a Promoter Gene in Clear Cell Renal Cell Carcinoma Cell Growth and Metastasis [Yu DP Med Sci Monit, 2018, 24:8213-8223] | PubMed: 30431025 |
| NFκB mediated elevation of KCNJ11 promotes tumor progression of hepatocellular carcinoma through interaction of lactate dehydrogenase A [Zhang K Biochem Biophys Res Commun, 2018, 495(1):246-253] | PubMed: 29108994 |
| Zinc finger RNA-binding protein promotes non-small-cell carcinoma growth and tumor metastasis by targeting the Notch signaling pathway [Zhang H Am J Cancer Res, 2017, 7(9):1804-1819] | PubMed: 28979805 |
| Dysregulation of the ADAM17/Notch signalling pathways in endometriosis: from oxidative stress to fibrosis [ Mol Hum Reprod, 2017, 23(7):488-499] | PubMed: 28486700 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.