FIDAS-3

Catalog No.E0081 Batch:E008101

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Technical Data

Formula

C₁₆H₁₅F₂N
Molecular Weight 259.29 CAS No. 1266684-01-8
Solubility (25°C)* In vitro DMSO 52 mg/mL (200.54 mM)
Ethanol 13 mg/mL (50.13 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description FIDAS-3 effectively competes against S-adenosylmethionine (SAM) for methionine sadenosyltransferase 2A (MAT2A) binding . FIDAS-3 has anticancer activities by inhibiting the expression of oncogenes. FIDAS-3 is a stilbene derivative and is a potent Wnt inhibitor with an IC50 of 4.9 μM for MAT2A.
Targets
hMat2A [1]
4.9 μM
In vitro

FIDAS-3 inhibits Methionine S-adenosyltransferase 2A (MAT2A) activity in S-adenosylmethionine (SAM) synthesis, leading to the inhibition of CRC cell growth.[1]
In vivo

FIDAS-3 represses the growth of human CRC xenografts in athymic nude mice with no notable effects on body weight.FIDAS-3 represses the growth of human CRC xenografts in athymic nude mice with no notable effects on body weight. [1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    LS174T cells

  • Concentrations

    3-10 μM

  • Incubation Time

    36 h

  • Method

    For cell based assay(Western blotting ; Cell proliferatio; shRNA assay), LS174T cells are cultured in RPMI1640 medium containing 5% FBS. Cells are treated with FIDAS agents for 36 h and then harvested and weighed. Perchloric acid (0.4 M) is added to cell pellet (100 μL/30mg) for deproteinization.
Animal Study:

[1]
  • Animal Models

    Athymic nude mice bearing with HT29 CRC cells

  • Dosages

    20 mg/kg

  • Administration

    o.g.

Selleck's FIDAS-3 has been cited by 2 publications

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment [ Cell Death Dis, 2023, 14(8):525] PubMed: 37582769
Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment [ Cell Death Dis, 2023, 14(8):525] PubMed: 37582769

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.