ex229 (compound 991)

Catalog No.S8654 Batch:S865401

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Technical Data

Formula

C24H18ClN3O3
Molecular Weight 431.87 CAS No. 1219739-36-2
Solubility (25°C)* In vitro DMSO 12 mg/mL (27.78 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
8%DMSO 40%PEG300＋5%Tween80＋47%ddH2O
1.16mg/ml Taking the 1 mL working solution as an example, add 80 μL of 14.5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 470 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description EX229 (compound 991) is a potent AMPK activator that is 5-10-fold more potent than A769662 in activating AMPK.
Targets
AMPK [1]
In vitro

EX229 activates AMPK in incubated rat epitrochlearis skeletal muscle and incubation of rat skeletal muscle with ex229 increases glucose uptake. The stimulation of skeletal muscle glucose uptake by ex229 is PI3K/PKB-independent, while AMPK-dependent. ex229 increases fatty acid oxidation and glucose uptake in L6 myotubes[1].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    HEK293T cells

  • Concentrations

    50 μM

  • Incubation Time

    1 h

  • Method

    Cells transfected with Myc-Rabep1 WT or Ser407Ala were treated for 60 min with vehicle or 50 μM 991.

Selleck's ex229 (compound 991) has been cited by 12 publications

Unexpected roles for AMPK in the suppression of autophagy and the reactivation of MTORC1 signaling during prolonged amino acid deprivation [ Autophagy, 2024, 1-24.] PubMed: 38744665
AMPK agonism optimizes the in vivo persistence and anti-leukemia efficacy of chimeric antigen receptor T cells [ bioRxiv, 2024, 2024.09.26.615290] PubMed: 39386600
CRISPR screens decode cancer cell pathways that trigger γδ T cell detection [ Nature, 2023, 621(7977):188-195] PubMed: 37648854
Redefining the role of AMPK in autophagy and the energy stress response [ Nat Commun, 2023, 14(1):2994] PubMed: 37225695
Activity-dependent subcellular compartmentalization of dendritic mitochondria structure in CA1 pyramidal neurons [ bioRxiv, 2023, 2023.03.25.534233] PubMed: 36993655
[Fuyu Decoction improves ventricular remodeling in rats with heart failure by inhibiting AMPK/mTOR pathway-mediated autophagy] [ Nan Fang Yi Ke Da Xue Xue Bao, 2023, 43(3):466-473] PubMed: 37087593
[Fuyu Decoction improves ventricular remodeling in rats with heart failure by inhibiting AMPK/mTOR pathway-mediated autophagy] [ Nan Fang Yi Ke Da Xue Xue Bao, 2023, 43(3):466-473] PubMed: 37087593
Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha [ Mol Cell, 2022, S1097-2765(22)01058-9] PubMed: 36384136
IL11 stimulates ERK/P90RSK to inhibit LKB1/AMPK and activate mTOR initiating a mesenchymal program in stromal, epithelial, and cancer cells [ iScience, 2022, 25(8):104806] PubMed: 35992082
Liver kinase B1 (LKB1) in murine erythroid progenitors modulates erythropoietin setpoint in association with maturation control [ Blood Cells Mol Dis, 2022, 97:102688] PubMed: 35717902

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.