Echinocystic acid

Catalog No.S3837 Batch:S383701

Technical Data

Formula	C₃₀H₄₈O₄
Molecular Weight	472.70	CAS No.	510-30-5
Solubility (25°C)*	In vitro	DMSO	94 mg/mL (198.85 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Echinocystic acid (EA), a natural triterpone enriched in various herbs, displays a range of pharmacological activities including anti-inflammatory and antioxidant effects.
In vitro	Echinocystic acid (EA) could induce apoptosis in human HepG2 cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. EA induces the truncation of Bid protein and reduction of Bcl-2 protein. EA also causes the loss of mitochondrial membrane potential (DWm) and cytochrome c release from mitochondria to cytosol. Moreover, EA could activate c-Jun NH2-terminal kinase (JNK) and p38 kinase, and JNK-specific inhibitor SP600125 and p38 kinase-specific inhibitor SB200235 could block serial molecular events of EA-induced apoptosis such as Bid truncation, Bcl-2 reduction, cytochrome c release, caspase activation, and DNA fragmentation in HepG2 cells. EA inhibits HepG2 cell proliferation in a dose-dependent manner with an IC50 value of 45.4 μM at 24h treatment^[1].
In vivo	Administration of Echinocystic acid (EA) is found to improve the maximum stress and Young’s modulus of femur in OVX rats. Micro-computed tomography analysis reveals that EA could improve the trabecular architecture, as shown by increasing the BV/TV, Tb.N, and Tb.Th in OVX rats. However, EA does not affect the body weight and uterine weight. EA has been shown to display an anti-inflammatory effect in different models of chronic inflammation in mice through the down-regulation of pro-inflammatory cytokines such as IL-1β, IL-18, TNF-α^[2]. EA attenuates reserpine-induced pain/depression dyad partially through regulating the biogenic amines levels and GluN2B receptors in the hippocampus^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HepG2 cells Concentrations 0, 15, 30, 45, 60, 100 μM Incubation Time 24h Method The cell survival rate is measured by a MTT assay. HepG2 cells (2 ×10⁴/well) are incubated with different concentrations of EA in 96-well plates for 24h. MTT solution (5 mg/ml) is then added to each well and incubated for another 4h. The resulting MTT-formazan product is dissolved by the addition of 0.04N HCl-isopropanol solutions. Absorbance is measured at 595 nm with a microplate reader. The cell viability is expressed as the optical density ratio of the treatment to control.
Animal Study:^{^[2]}	Animal Models female ovariectomy (OVX) Sprague–Dawley rats Dosages 1, 5 or 15 mg/kg/day Administration ig

Cell Assay:^{^[1]}

Cell lines
HepG2 cells
Concentrations
0, 15, 30, 45, 60, 100 μM
Incubation Time
24h
Method
The cell survival rate is measured by a MTT assay. HepG2 cells (2 ×10⁴/well) are incubated with different concentrations of EA in 96-well plates for 24h. MTT solution (5 mg/ml) is then added to each well and incubated for another 4h. The resulting MTT-formazan product is dissolved by the addition of 0.04N HCl-isopropanol solutions. Absorbance is measured at 595 nm with a microplate reader. The cell viability is expressed as the optical density ratio of the treatment to control.

Animal Study:^{^[2]}

Animal Models
female ovariectomy (OVX) Sprague–Dawley rats
Dosages
1, 5 or 15 mg/kg/day
Administration
ig

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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