Echinacoside

Catalog No.S3783 Batch:S378301

Technical Data

Formula	C₃₅H₄₆O₂₀
Molecular Weight	786.73	CAS No.	82854-37-3
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (127.1 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Echinacoside, a natural polyphenolic compound, has various biological activities, such as antioxidative, anti-inflammatory, neuroprotective, hepatoprotective, nitric oxide radical-scavenging and vasodilative actions. Echinacoside, one of the phenylethanoids isolated from the stems of Cistanche salsa, elicits neuroprotection by activating Trk receptors and their downstream signal pathways. Echinacoside also has a remarkable antiosteoporotic activity.
In vitro	Echinacoside behaves in vitro as an anti-oxidant and a potent free radical scavenger. It has been shown to rescue SH-SY5Y neuronal cells from TNF-alpha-induced apoptosis. Echinacoside significantly attenuates glutamate-induced neurotoxicity and exhibits neuroprotective activities in vitro^[1]. Echinacoside has significant neuroprotective effects on TNFα-induced apoptosis in SHSY5Y neuronal cells by maintaining mitochondrial function, decreasing the generation of reactive oxygen species, increasing the expression of the antiapoptotic protein Bcl-2 and inhibiting caspase-3 activity through an antioxidation mechanism^[2].
In vivo	Echinacoside has neuroprotective effects in the mouse MPTP model of Parkinson's disease. Treatment with echinacoside improves motor behavior and attenuated the depletion of striatal dopamine, DOPAC and HVA in MPTP-lesioned mice^[1].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines SHSY5Y cells Concentrations 1, 10 or 100 μg/ml Incubation Time 36 h Method Cell viability is determined by use of an MTT assay. Cells are seeded in 96-well plates at 1×10⁴ cells per well and grown to 70% confluence in culture medium. The medium is replaced by medium containing various concentrations of echinacoside (1, 10 or 100 μg/ml) and/or 100 ng/ml TNFα. A total of 5 mg/ml MTT is added to each well after 36 h, and the culture continued to incubate for another 4 h at 37 °C. After the medium has been removed, cells and dye crystals are solubilized with 200 μl dimethylsulfoxide (DMSO), and optical density is measured at 570 nm.
Animal Study:^{^[1]}	Animal Models Adult male C57BL/6 mice Dosages 5 and 20 mg/kg Administration p.o.

Cell Assay:^{^[2]}

Cell lines
SHSY5Y cells
Concentrations
1, 10 or 100 μg/ml
Incubation Time
36 h
Method
Cell viability is determined by use of an MTT assay. Cells are seeded in 96-well plates at 1×10⁴ cells per well and grown to 70% confluence in culture medium. The medium is replaced by medium containing various concentrations of echinacoside (1, 10 or 100 μg/ml) and/or 100 ng/ml TNFα. A total of 5 mg/ml MTT is added to each well after 36 h, and the culture continued to incubate for another 4 h at 37 °C. After the medium has been removed, cells and dye crystals are solubilized with 200 μl dimethylsulfoxide (DMSO), and optical density is measured at 570 nm.

Animal Study:^{^[1]}

Animal Models
Adult male C57BL/6 mice
Dosages
5 and 20 mg/kg
Administration
p.o.

References

Selleck's Echinacoside has been cited by 2 publications

Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis [ Antioxidants (Basel), 2023, 10.3390/antiox12111925]	PubMed: 38001778
Astragaloside IV and echinacoside benefit neuronal properties via direct effects and through upregulation of SOD1 astrocyte function in vitro [ Naunyn Schmiedebergs Arch Pharmacol, 2020, 10.1007/s00210-020-02022-w]	PubMed: 33219470

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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