Dp44mT

Catalog No.S7909 Batch:S790901

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Technical Data

Formula

C14H15N5S
Molecular Weight 285.37 CAS No. 152095-12-0
Solubility (25°C)* In vitro DMSO 57 mg/mL (199.74 mM)
Ethanol 47 mg/mL (164.69 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Dp44mT is a potent iron chelator, which shows selective antitumor activity.
Targets
iron [1]
In vitro Dp44mT shows pronounced antiproliferative effects in SK-N-MC, SK-Mel-28, and MCF-7 cells with IC50 of 30 nM, 60 nM, and 60 nM, respectively, while no effects on normal MRC-5 fibroblasts. Dp44mT inhibits cellular Fe uptake from Fe-Tf in SK-N-MC neuroepithelioma and M109 cells, and induces cell apoptosis. [1] In MDA-MB-231 cells, Dp44mT specifically targets topoisomerase topo2α, and thus causes DNA damage. [2] Dp44mT, as a Pgp substrate, also overcomes multidrug resistance by the hijacking of lysosomal P-glycoprotein (Pgp). [3]
In vivo In CD2F1 mice bearing M109 tumors, Dp44mT (0.4 mg/kg, i.v.) inhibits tumor growth in a dose-dependent manner. [1]

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    SK-N-MC, SK-Mel-28, MCF-7 and MRC-5 cells

  • Concentrations

    ~25 μM

  • Incubation Time

    72 h

  • Method

    Cells are incubated in the presence and absence of DFO, 311, 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, doxorubicin, and the DpT series of chelators (0-25 μM) for 72 hours at 37°C。 The effect of the chelators on proliferation is examined using the MTT assay.
Animal Study:[1]
  • Animal Models

    CD2F1 mice bearing M109 tumors

  • Dosages

    04 mg/kg, twice daily

  • Administration

    i.v.

Selleck's Dp44mT has been cited by 4 publications

Curcumin Analog, HO-3867, Induces Both Apoptosis and Ferroptosis via Multiple Mechanisms in NSCLC Cells with Wild-Type p53 [ Evid Based Complement Alternat Med, 2023, 2023:8378581] PubMed: 36814470
Iron Deficiency Increases Phosphorylation of SP1 to Upregulate SPNS2 Expression in Hepatocellular Carcinoma [ Biol Trace Elem Res, 2022, 10.1007/s12011-022-03296-2] PubMed: 35614326
Sphingosine-1-phosphate transporter spinster homolog 2 is essential for iron-regulated metastasis of hepatocellular carcinoma [ Mol Ther, 2021, S1525-0016(21)00467-6] PubMed: 34547466
Impaired-ferritinophagy-flux-induced-by-high-fat-diet-mediates-hepatic-insulin-resistance-via-endoplasmic-reticulum-stress. [ Food-Chem-Toxicol, 2020, 10;140:111329] PubMed: 32283200

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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