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Technical Data
| Formula | C22H24N2O8.HCl |
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| Molecular Weight | 480.9 | CAS No. | 10592-13-9 | |
| Solubility (25°C)* | In vitro | DMSO | 3 mg/mL (6.23 mM) | |
| Water | Insoluble | |||
| Ethanol | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Doxycycline HCl is a tetracycline antibiotic that commonly used to treat a variety of infections, and also a MMP inhibitor. | |
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| Targets |
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| In vitro | Doxycycline (50 μM) completely inhibits degradation of type II collagen, but not that of type I collagen in rheumatoid synovial fibroblasts and endothelial cells. Doxycycline (50 μM) down-regulated MMP-8 induction, at both the mRNA and protein levels in rheumatoid synovial fibroblasts and endothelial cells. [1] Doxycycline not only inhibits MMP-8 and MMP-9 (gelatinase B) activity, but also the synthesis of MMPs in human endothelial cells. Doxycycline (50 μM) completely inhibits the phorbol-12-myristate-13-acetate (PMA)-mediated induction of MMP-8 and MMP-9, as measured by Western blotting and gelatin zymography, respectively. [2] Doxycycline inhibits the LPS-induced IL-1beta increase in the mRNA and protein amounts in the corneal epithelium and upregulates the expression of the anti-inflammatory IL-1 RA protein in human cultured corneal epithelium. Doxycycline reduces the steady state level of the cellular ICE protein but does not affect the level of ICE transcripts. Doxycycline significantly decreases IL-1beta bioactivity in the supernatants from LPS-treated corneal epithelial cultures.[3] | |
| In vivo | Doxycycline (25 mg/day) prevents the structural deterioration of aortic elastin without decreasing the influx of inflammatory cells in Wistar rats. Doxycycline (25 mg/day) markedly suppresses increased aortic wall production of 92 kD gelatinase in Wistar rats. [4] Doxycycline reduces the activity of matrix metalloproteinase (MMP)-2 and MMP-9 in the arterial wall, and inhibits smooth muscle cell migration from media to intima by 77% at 4 days after balloon injury in rats. Doxycycline reduces the replication of smooth muscle cells in the intima in rats. [5] |
Protocol (from reference)
References
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.