Doxycycline

Catalog No.S5159 Batch:S515904

Technical Data

Formula	C₂₂H₂₄N₂O₈
Molecular Weight	444.43	CAS No.	564-25-0
Solubility (25°C)*	In vitro	DMSO	89 mg/mL (200.25 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.
In vitro	100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used^[1]. Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes^[2].
In vivo	Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)^[2].

Description

Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.

In vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used^[1].

Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes^[2].

In vivo

Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines Concentrations 100 ng/mL, 1 µg/mL Incubation Time 24, 72, and 96 hours Method Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.
Animal Study:^{^[2]}	Animal Models Male nu/nu mice Dosages 35 mg/pellet Administration s.c.

Cell Assay:^{^[1]}

Cell lines
U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
Concentrations
100 ng/mL, 1 µg/mL
Incubation Time
24, 72, and 96 hours
Method
Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.

Animal Study:^{^[2]}

Animal Models
Male nu/nu mice
Dosages
35 mg/pellet
Administration
s.c.

References

Selleck's Doxycycline has been cited by 47 publications

Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human [ Nat Genet, 2024, 56(1):124-135]	PubMed: 38195860
Destabilization of fear memory by Rac1-driven engram-microglia communication in hippocampus [ Brain Behav Immun, 2024, 119:621-636]	PubMed: 38670239
The AKR1C1-CYP1B1-cAMP signaling axis controls tumorigenicity and ferroptosis susceptibility of extrahepatic cholangiocarcinoma [ Cell Death Differ, 2024, ]	PubMed: 39478199
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137]	PubMed: 38642502
Ubiquitin-specific protease 22 controls melanoma metastasis and vulnerability to ferroptosis through targeting SIRT1/PTEN/PI3K signaling [ MedComm (2020), 2024, 5(8):e684]	PubMed: 39135915
UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis [ Cell Rep, 2024, 43(2):113808]	PubMed: 38367236
Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER [ J Cell Biol, 2024, 223(10)e202304031]	PubMed: 39133213
DNp73 enhances tumor progression and immune evasion in multiple myeloma by targeting the MYC and MYCN pathways [ Front Immunol, 2024, 15:1470328]	PubMed: 39380995
YB-1 regulates mesothelioma cell migration via snail but not EGFR, MMP1, EPHA5 or PARK2 [ Mol Oncol, 2024, 18(4):815-831]	PubMed: 36550787
The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation [ J Biol Chem, 2024, 300(3):105671]	PubMed: 38272222

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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