Doxycycline

Catalog No.S5159 Batch:S515903

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Technical Data

Formula

C22H24N2O8

Molecular Weight 444.43 CAS No. 564-25-0
Solubility (25°C)* In vitro DMSO 89 mg/mL (200.25 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
3.25mg/ml Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.
In vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used[1].

Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes[2].

In vivo

Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)[2].

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines

  • Concentrations

    100 ng/mL, 1 µg/mL

  • Incubation Time

    24, 72, and 96 hours

  • Method

    Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.

Animal Study:

[2]

  • Animal Models

    Male nu/nu mice

  • Dosages

    35 mg/pellet

  • Administration

    s.c.

Selleck's Doxycycline has been cited by 47 publications

Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human [ Nat Genet, 2024, 56(1):124-135] PubMed: 38195860
Destabilization of fear memory by Rac1-driven engram-microglia communication in hippocampus [ Brain Behav Immun, 2024, 119:621-636] PubMed: 38670239
The AKR1C1-CYP1B1-cAMP signaling axis controls tumorigenicity and ferroptosis susceptibility of extrahepatic cholangiocarcinoma [ Cell Death Differ, 2024, ] PubMed: 39478199
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
Ubiquitin-specific protease 22 controls melanoma metastasis and vulnerability to ferroptosis through targeting SIRT1/PTEN/PI3K signaling [ MedComm (2020), 2024, 5(8):e684] PubMed: 39135915
UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis [ Cell Rep, 2024, 43(2):113808] PubMed: 38367236
Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER [ J Cell Biol, 2024, 223(10)e202304031] PubMed: 39133213
DNp73 enhances tumor progression and immune evasion in multiple myeloma by targeting the MYC and MYCN pathways [ Front Immunol, 2024, 15:1470328] PubMed: 39380995
YB-1 regulates mesothelioma cell migration via snail but not EGFR, MMP1, EPHA5 or PARK2 [ Mol Oncol, 2024, 18(4):815-831] PubMed: 36550787
The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation [ J Biol Chem, 2024, 300(3):105671] PubMed: 38272222

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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