Dooku1

Catalog No.S2953 Batch:S295301

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Technical Data

Formula

C13H9Cl2N3OS
Molecular Weight 326.20 CAS No. 2253744-54-4
Solubility (25°C)* In vitro DMSO 65 mg/mL (199.26 mM)
Ethanol 5 mg/mL (15.32 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
1.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Dooku1 is an analog of Yoda1 with antagonist activity against mechanosensitive Piezo1 channel. Dooku1 inhibits 2 μM Yoda1-induced Ca2+-entry with IC50 of 1.3 μM in HEK 293 cells and 1.5 μM in HUVECs, respectively.
Targets
Ca2+-entry [1]
(in HEK 293 cells)		 Ca2+-entry [1]
(in HUVECs)
1.3 μM 1.5 μM
In vitro

Dooku1 inhibits 2 μM Yoda1-induced Ca2+ -entry with IC50 s of 1.3 μM (HEK 293 cells) and 1.5 μM (HUVECs) yet failed to inhibit constitutive Piezo1 channel activity. Dooku1 has no effect on endogenous ATP-evoked Ca2+ elevation or store-operated Ca2+ entry in HEK 293 cells or Ca2+ entry through TRPV4 or TRPC4 channels overexpressed in CHO and HEK 293 cells.

Protocol (from reference)

Selleck's Dooku1 has been cited by 1 publication

Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis [ Sci Adv, 2023, 9(22):eadg0478] PubMed: 37267365

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.