DNase I, Bovine pancreas

Catalog No.E7018

Technical Data

Formula
Molecular Weight	N/A	CAS No.	9003-98-9
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description	DNase I, Bovine pancreas is a Ca²⁺/Mg²⁺-dependent endonuclease that cleaves phosphodiester bonds double-stranded DNA, classified under EC 3.1.21.1 as a hydrolase. It binds to the minor groove of DNA, with key amino acids like R111, Y211, and N170 interacting with the DNA backbone to facilitate catalysis, while residues N74 and S206 in the active site are crucial for substrate binding and DNA recognition. DNase I is essential for DNA degradation, chromatin research, and is widely used to generate smaller DNA fragments for genomic studies and to examine DNA-protein interactions in chromatin remodeling research.

Description

DNase I, Bovine pancreas is a Ca²⁺/Mg²⁺-dependent endonuclease that cleaves phosphodiester bonds double-stranded DNA, classified under EC 3.1.21.1 as a hydrolase. It binds to the minor groove of DNA, with key amino acids like R111, Y211, and N170 interacting with the DNA backbone to facilitate catalysis, while residues N74 and S206 in the active site are crucial for substrate binding and DNA recognition. DNase I is essential for DNA degradation, chromatin research, and is widely used to generate smaller DNA fragments for genomic studies and to examine DNA-protein interactions in chromatin remodeling research.

Protocol (from reference)

References

[1] Evans SJ, et al. Biochemistry. 1999 Mar 30;38(13):3902-9.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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