DL-Serine

Catalog No.S5545 Batch:S554501

Technical Data

Formula	C₃H₇NO₃
Molecular Weight	105.09	CAS No.	302-84-1
Solubility (25°C)*	In vitro	Water	8 mg/mL (76.12 mM)
		DMSO	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Serine is a non-essential amino acid and a natural ligand and allosteric activator of pyruvate kinase M2.
In vitro	The non-essential amino acid serine supports several metabolic processes that are crucial for the growth and survival of proliferating cells, including protein, amino acid and glutathione synthesis. As an important one-carbon donor to the folate cycle, serine contributes to nucleotide synthesis, methylation reactions and the generation of NADPH for antioxidant defence^[2]. Serine activates recombinant PKM2 with a half maximal activation concentration (AC50) of 1.3 mM. Isothermal titration calorimetry was used to determine the dissociation constant (Kd) of the PKM2-serine interaction as 0.20 mM. Serine can bind to and activate human PKM2 and that following serine deprivation, PKM2 activity in cells is reduced. This reduction in PKM2 activity shifts cells to a fuel-efficient mode where more pyruvate is diverted to the mitochondria and more glucose derived carbon is channelled into serine biosynthesis to support cell proliferation^[1].

Description

Serine is a non-essential amino acid and a natural ligand and allosteric activator of pyruvate kinase M2.

In vitro

The non-essential amino acid serine supports several metabolic processes that are crucial for the growth and survival of proliferating cells, including protein, amino acid and glutathione synthesis. As an important one-carbon donor to the folate cycle, serine contributes to nucleotide synthesis, methylation reactions and the generation of NADPH for antioxidant defence^[2]. Serine activates recombinant PKM2 with a half maximal activation concentration (AC50) of 1.3 mM. Isothermal titration calorimetry was used to determine the dissociation constant (Kd) of the PKM2-serine interaction as 0.20 mM. Serine can bind to and activate human PKM2 and that following serine deprivation, PKM2 activity in cells is reduced. This reduction in PKM2 activity shifts cells to a fuel-efficient mode where more pyruvate is diverted to the mitochondria and more glucose derived carbon is channelled into serine biosynthesis to support cell proliferation^[1].

Protocol (from reference)

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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