DKM 2-93

Catalog No.S5841 Batch:S584101

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Technical Data

Formula

C11H14ClNO3
Molecular Weight 243.69 CAS No. 65836-72-8
Solubility (25°C)* In vitro DMSO 49 mg/mL (201.07 mM)
Ethanol 14 mg/mL (57.45 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description DKM 2-93 is a relatively selective lead inhibitor of ubiquitin-like modifier activating enzyme 5 (UBA5).
Targets
UBA5 [1]
In vitro DKM 2-93 not only impairs pancreatic cancer cell survival in PaCa2, but also Panc1 cells with 50 % effective concentration values of 90 and 30 μM, respectively[1].
In vivo DKM 2-93 daily treatment significantly impairs tumor growth of PaCa2 cells in vivo in tumor xenograft studies in immune-deficient mice without causing any weight loss or overt toxicity[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    PaCa2 and Panc1 pancreatic cancer cells

  • Concentrations

    --

  • Incubation Time

    48 h

  • Method

    Cells are plated the evening before the experiment, and allowed to adhere overnight. For both survival and proliferation assays, cells are plated in regular media. Before dosing, the media is aspirated from all wells and replaced with the appropriate media and drug dosage. For the chemical genetics screen, cells are treated with either DMSO or the cysteine-reactive fragment for 48 h and cell viability is assessed by Hoescht stain.
Animal Study:

[1]
  • Animal Models

    C.B17 SCID male mice (6-8 weeks old)

  • Dosages

    50 mg/kg

  • Administration

    i.p.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.