Diflunisal

Catalog No.S4609 Batch:S460901

Technical Data

Formula	C₁₃H₈F₂O₃
Molecular Weight	250.20	CAS No.	22494-42-4
Solubility (25°C)*	In vitro	DMSO	50 mg/mL (199.84 mM)
		Ethanol	20 mg/mL (79.93 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Diflunisal (Dolobid, Dolobis, Flovacil, Fluniget,MK-647) is a difluorophenyl derivate of salicylic acid and a nonsteroidal anti-inflammatory drug (NSAID) with antipyretic, analgesic and anti-inflammatory properties. The mechanism of action of diflunisal is as a Cyclooxygenase Inhibitor.

Targets

COX ^[1]	p300 ^[2]
	996 μM

In vitro

Diflunisal, an FDA-approved drug containing a salicylic acid substructure, inhibited CBP/p300 more potently than salicylate. Diflunisal exhibits anti-tumor activity against a specific leukemia carrying a t(8;21) translocation, a tumor previously reported to be dependent on p300 in vitro and in vivo^[2].

In vivo

Diflunisal-mediated kinetic stabilization of TTR(Rate-limiting transthyretin) should ameliorate TTR amyloidoses, provided that the nonsteroidal anti-inflammatory drug liabilities can be managed clinically^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HEK293T cells Concentrations 0, 100, 200, 400 μM Incubation Time 24 h Method HEK293T cells are transfected with expression vectors for WT p300 or catalitycally inactive mutant. 24 hr after transfection, cells are treated with diflunisal as indicated for 24 hr. H2B acetylation is measured by Western Blot using specified antibodies. Experiments are repeated four times.
Animal Study:^{^[1]}	Animal Models Female Sprague-Dawley rats, Female New Zealand White Rabbits Dosages 250 mg/kg Administration oral administration

Cell Assay:^{^[2]}

Cell lines
HEK293T cells
Concentrations
0, 100, 200, 400 μM
Incubation Time
24 h
Method
HEK293T cells are transfected with expression vectors for WT p300 or catalitycally inactive mutant. 24 hr after transfection, cells are treated with diflunisal as indicated for 24 hr. H2B acetylation is measured by Western Blot using specified antibodies. Experiments are repeated four times.

Animal Study:^{^[1]}

Animal Models
Female Sprague-Dawley rats, Female New Zealand White Rabbits
Dosages
250 mg/kg
Administration
oral administration

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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