Desmopressin Acetate

Catalog No.P1084 Batch:P108401

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Technical Data

Formula

C46H64N14O12S2.C2H4O2
Molecular Weight 1129.27 CAS No. 62288-83-9
Solubility (25°C)* In vitro DMSO 100 mg/mL (88.55 mM)
Water 100 mg/mL (88.55 mM)
Ethanol 100 mg/mL (88.55 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Desmopressin is a synthetic octapeptide, and an analogue of human hormone arginine vasopressin with antidiuretic and coagulant activities. It is a selective agonist for the vasopressin V2 receptor (V2R).
In vitro Desmopressin (DDAVP) is a selective agonist for the vasopressin V2 receptor (V2R), which is coupled to cAMP-dependent signaling. DDAVP induces cAMP-dependent eNOS activation via Ser1177 phosphorylation[1]. DDAVP (at doses ranging from 100 ng/ml to 1 μg/ml) exerts a modest but significant antiproliferative effect on cultured CT-26 and Colo-205 cells[2].
In vivo In vivo, DDAVP (2 μg/kg) reduces accumulation of ascites and formation of intestinal tumor nodules in mice intraperitoneally inoculated with CT-26 cells. Perioperative administration of DDAVP significantly inhibits tumor progression in animals surgically implanted in the spleen with CT-26 cells, and causes some reduction in liver metastasis. DDAVP is able to inhibit lung colonization by blood-borne breast cancer cells in an experimental murine model. It dramatically reduces lymph node and lung metastasis in a model of mammary tumor manipulation and surgical excision in mice when administered perioperatively, and also exerts antitumor effects in combination with chemotherapy[2]. DDAVP increases the plasma levels of coagulation factor VIII, von Willebrand factor (VWF) and tissue-type plasminogen activator, and also enhances platelet adhesion to the vessel wall[1].
Density 95.0~105.0%

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HUVECs

  • Concentrations

    1 μM

  • Incubation Time

    25 min

  • Method

    HUVECs are transduced with recombinant adenoviruses AdV2R.EGFP (AdV2R) or AdlacZ. 3 days later cells are incubated for 25 min with 1 μM DDAVP or 10 μM forskolin, both in presence of IBMX. Cell extracts are analyzed for eNOS Ser1177 phosphorylation and eNOS expression.
Animal Study:

[2]
  • Animal Models

    Syngeneic adult male Balb/c mice inoculated intraperitoneally or intrasplenically with CT-26 colon cancer cells

  • Dosages

    2 μg/kg body weight

  • Administration

    i.v.

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.