Deracoxib

Catalog No.S5711 Batch:S571101

Technical Data

Formula	C₁₇H₁₄F₃N₃O₃S
Molecular Weight	397.37	CAS No.	169590-41-4
Solubility (25°C)*	In vitro	DMSO	80 mg/mL (201.32 mM)
		Ethanol	20 mg/mL (50.33 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Deracoxib (SC-046) is a COX-2 inhibitor with >48-fold selectivity over COX-1. It also exhibits inhibitory activity against phosphodiesterase (Ki = 3.6 μM against human PDE4D3).

Targets

COX2 ^[1]	PDE4 ^[2] (Cell-free assay)
	3.6 μM

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MC4R-GLO cells Concentrations 10 µM Incubation Time 5 min Method MC4R-GLO cells were plated in 12 well culture dishes in DMEM/F12 supplemented with 5% fetal bovine serum. Cells were incubated in serum-free medium over night before the experiment. Cells were then treated with the indicated concentration of α-MSH or vehicle for 5 min at 37°C in the presence or absence of 10 µM deracoxib or imperatorin. Dishes were then placed on ice and cells were scraped in 1x LDS-PAGE sample buffer with 100 mM DTT. Proteins were resolved by SDS-PAGE.

Cell Assay:^{^[2]}

Cell lines
MC4R-GLO cells
Concentrations
10 µM
Incubation Time
5 min
Method
MC4R-GLO cells were plated in 12 well culture dishes in DMEM/F12 supplemented with 5% fetal bovine serum. Cells were incubated in serum-free medium over night before the experiment. Cells were then treated with the indicated concentration of α-MSH or vehicle for 5 min at 37°C in the presence or absence of 10 µM deracoxib or imperatorin. Dishes were then placed on ice and cells were scraped in 1x LDS-PAGE sample buffer with 100 mM DTT. Proteins were resolved by SDS-PAGE.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.