Dendrophenol

Catalog No.S6370 Batch:S637001

Technical Data

Formula	C₁₇H₂₀O₅
Molecular Weight	304.34	CAS No.	108853-14-1
Solubility (25°C)*	In vitro	DMSO	61 mg/mL (200.43 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Dendrophenol (Moscatilin) is from Dendrobium nobile, a naturally occurring bibenzyl compound with potential antimutagenic activity. Dendrophenol (Moscatilin) almost completely suppresses (97%) the AFB1-induced SOS response with an IC50 of 0.08 μM.
In vitro	Dendrophenol induces apoptosis of colorectal HCT-116 cells via tubulin depolymerization and DNA damage stress and that this leads to the activation of JNK and mitochondria-involved intrinsic apoptosis pathway.^[2]
In vivo	In Male severe combined immunodeficient mice implanted with HCT-116 cells, the i.p. treatment with dendrophenol causes a significant inhibition of tumor growth without the loss of body weight.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HCT-116 cells Concentrations 3, 10, 30 μM Incubation Time 30 min Method The CytoDYNAMIX ScreenTM3 (CDS-03) kits are used for the detection of polymerization of tubulin/microtubule. Tubulin proteins (>99% purity) are suspended (300 μg/sample) with 100 μL G-PEM buffer plus 5% glycerol in the absence or presence of dendrophenol at 4 ℃. Then, the sample mixture is transferred to the 96-well plate, and the polymerization of tubulin is measured at 340 nm every 0.5 min for 45 min at 37 ℃.

Cell Assay:^{^[2]}

Cell lines
HCT-116 cells
Concentrations
3, 10, 30 μM
Incubation Time
30 min
Method
The CytoDYNAMIX ScreenTM3 (CDS-03) kits are used for the detection of polymerization of tubulin/microtubule. Tubulin proteins (>99% purity) are suspended (300 μg/sample) with 100 μL G-PEM buffer plus 5% glycerol in the absence or presence of dendrophenol at 4 ℃. Then, the sample mixture is transferred to the 96-well plate, and the polymerization of tubulin is measured at 340 nm every 0.5 min for 45 min at 37 ℃.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.