Dehydroevodiamine hydrochloride

Catalog No.S3820 Batch:S382001

Technical Data

Formula	C₁₉H₁₅N₃O.HCl
Molecular Weight	337.8	CAS No.	111664-82-5
Solubility (25°C)*	In vitro	DMSO	37 mg/mL (109.53 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Dehydroevodiamine (DHE), a major phytochemical in fruits of Evodia rutaecarpa, can inhibit AChE. It has hypotensive and neuroprotective effects and modulates nitric oxide production.

Targets

AChE ^[2]

In vitro

Dehydroevodiamine·HCl (DHED) treatment significantly recovers the decreases in the levels of neural cell adhesion molecule (NCAM) proteins caused by stress and the decreases in cell viability^[1]. It decreases intracellular calcium levels in primary cortical neurons^[2].

In vivo

Dehydroevodiamine·HCl (DHED) can improve stress-induced memory impairments and depression-like behaviors^[1]. DHED significantly improves learning and memory in the Aβ_1-42-infused rat model. Antiamnestic effects of DHED occur through an improvement of learning and memory and an inhibition of neuronal dysfunction in a scopolamine-induced amnesic rat model. Furthermore, Dehydroevodiamine·HCl (DHED) has hypotensive and neuroprotective effects and modulates nitric oxide production^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines primary cortical neurons Concentrations 0.5 and 1 µM Incubation Time 4 h Method Cells are cultured in a specified medium for neurons (neurobasal medium supplemented with B27 and penicillin-streptomycin (100 U/ml/100 mg/ml) at 37℃ and 5% CO2 and plated at a density of 5×10⁴ cells/well on Deckgläser cover glasses coated with PLL (1 mg/ml) in the 12 well plates. Experiments are performed on 10-day cultures. After DHED (0.5 and 1 µM) and fluoxetine (0.5 µM) treatment for 4 hr, staurosporin (1 µM) are added for 1 hr.
Animal Study:^{^[1]}	Animal Models male Wistar rats Dosages 10 mg/kg/day Administration p.o.

Cell Assay:^{^[1]}

Cell lines
primary cortical neurons
Concentrations
0.5 and 1 µM
Incubation Time
4 h
Method
Cells are cultured in a specified medium for neurons (neurobasal medium supplemented with B27 and penicillin-streptomycin (100 U/ml/100 mg/ml) at 37℃ and 5% CO2 and plated at a density of 5×10⁴ cells/well on Deckgläser cover glasses coated with PLL (1 mg/ml) in the 12 well plates. Experiments are performed on 10-day cultures. After DHED (0.5 and 1 µM) and fluoxetine (0.5 µM) treatment for 4 hr, staurosporin (1 µM) are added for 1 hr.

Animal Study:^{^[1]}

Animal Models
male Wistar rats
Dosages
10 mg/kg/day
Administration
p.o.

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.