Deferoxamine mesylate

Catalog No.S5742 Batch:S574209

Technical Data

Formula	C₂₆H₅₂N₆O₁₁S
Molecular Weight	656.79	CAS No.	138-14-7
Solubility (25°C)*	In vitro	Water	100 mg/mL (152.25 mM)
		DMSO	50 mg/mL (76.12 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Deferoxamine mesylate is the mesylate salt of Deferoxamine, which forms iron complexes and is used as a chelating agent. Deferoxamine is a ferroptosis inhibitor that stabilizes HIF-1α expression and improves HIF-1α transactivity in hypoxic and hyperglycemic states in vitro. Deferoxamine decreases beta-amyloid (Aβ) deposition and induces autophagy.Please do not prepare stock solutions with normal saline or PBS, as precipitation may occur.

Targets

HIF-1α ^[1]

Beta Amyloid ^[1]

Ferroptosis ^[2]

In vitro

Deferoxamine mesylate, the iron chelator and ferroptosis inhibitor, rescues neutrophil death induced by systemic lupus erythematosus (SLE) serum, suggesting that ferroptosis may be the main form of neutrophil death in SLE.^[3]

In vivo

Deferoxamine mesylate (Ba 33112, Desferrioxamine B, DFOM, NSC 644468) is the mesylate salt of Deferoxamine, which forms iron complexes and is used as a chelating agent.

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines Healthy neutrophil cell Concentrations 1/10/100 μM Incubation Time 16 h Method Cells are cultured in complete RPMI 1640 basic medium (Gibco) with 100 U/ml penicillin, 100 μg/ml streptomycin and 20% serum from ten randomly selected patients with SLE or age- and sex-matched healthy controls (HCs), and incubated at 37 ℃ in a humidified atmosphere of 20% O₂ and 5% CO₂. HC neutrophil cell viability treated with two ferroptosis inhibitors, LPX-1 (10/100/1,000 nM) or DFO (1/10/100 μM), two necroptosis inhibitors, Nec-1 (10/100/1,000 nM) or NSA (10/100/1,000 nM), or apoptosis inhibitor Z-VAD (0.1/1/10 μM) for 16 h are assessed by flow cytometry.
Animal Study:^{^[4]}	Animal Models Mongrel dogs Dosages 25 mg/kg Administration i.m.

Cell Assay:^{^[3]}

Cell lines
Healthy neutrophil cell
Concentrations
1/10/100 μM
Incubation Time
16 h
Method
Cells are cultured in complete RPMI 1640 basic medium (Gibco) with 100 U/ml penicillin, 100 μg/ml streptomycin and 20% serum from ten randomly selected patients with SLE or age- and sex-matched healthy controls (HCs), and incubated at 37 ℃ in a humidified atmosphere of 20% O₂ and 5% CO₂. HC neutrophil cell viability treated with two ferroptosis inhibitors, LPX-1 (10/100/1,000 nM) or DFO (1/10/100 μM), two necroptosis inhibitors, Nec-1 (10/100/1,000 nM) or NSA (10/100/1,000 nM), or apoptosis inhibitor Z-VAD (0.1/1/10 μM) for 16 h are assessed by flow cytometry.

Animal Study:^{^[4]}

Animal Models
Mongrel dogs
Dosages
25 mg/kg
Administration
i.m.

References

[4] Sanan S, et al. Resuscitation. 1989 Feb;17(1):63-75.

+ Expand

Selleck's Deferoxamine mesylate has been cited by 91 publications

Tryptophan 2,3-dioxygenase-positive matrix fibroblasts fuel breast cancer lung metastasis via kynurenine-mediated ferroptosis resistance of metastatic cells and T cell dysfunction [ Cancer Commun (Lond), 2024, 10.1002/cac2.12608]	PubMed: 39221971
Pyroptotic T cell-derived active IL-16 has a driving function in ovarian endometriosis development [ Cell Rep Med, 2024, 5(3):101476]	PubMed: 38508138
Enterovirus-A71 preferentially infects and replicates in human motor neurons, inducing neurodegeneration by ferroptosis [ Emerg Microbes Infect, 2024, 13(1):2382235]	PubMed: 39017655
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R [ Redox Biol, 2024, 72:103154]	PubMed: 38626575
Heterogeneous ferroptosis susceptibility of macrophages caused by focal iron overload exacerbates rheumatoid arthritis [ Redox Biol, 2024, 69:103008]	PubMed: 38142586
The involvement of the Stat1/Nrf2 pathway in exacerbating Crizotinib-induced liver injury: implications for ferroptosis [ Cell Death Dis, 2024, 15(8):600]	PubMed: 39160159
Mitochondrial GCN5L1 acts as a novel regulator for iron homeostasis to promote sorafenib sensitivity in hepatocellular carcinoma [ J Transl Med, 2024, 22(1):593]	PubMed: 38918793
Analysis of High-Dose Ascorbate-Induced Cytotoxicity in Human Glioblastoma Cells and the Role of Dehydroascorbic Acid and Iron [ Antioxidants (Basel), 2024, 13(9)1095]	PubMed: 39334754
Silica nanoparticles induce ferroptosis of HUVECs by triggering NCOA4-mediated ferritinophagy [ Ecotoxicol Environ Saf, 2024, 270:115889]	PubMed: 38150751
Oncolytic Newcastle disease virus induced degradation of YAP through E3 ubiquitin ligase PRKN to exacerbate ferroptosis in tumor cells [ J Virol, 2024, 98(3):e0189723]	PubMed: 38411946

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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