DC661

Catalog No.S8808 Batch:S880801

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Technical Data

Formula

C31H39Cl2N5
Molecular Weight 552.58 CAS No. 1872387-43-3
Solubility (25°C)* In vitro Ethanol 49 mg/mL (88.67 mM)
DMSO 7 mg/mL (12.66 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
2%DMSO 40%PEG300 5%Tween80 53%ddH2O
0.2mg/ml Taking the 1 mL working solution as an example, add 20 μL of clarified DMSO stock solution of 10 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description DC661 is capable of deacidifying the lysosome and inhibiting autophagy significantly better than HCQ. DC661 induces apoptosis.
Targets
Autophagy [1]
In vitro

Treatment of melanoma cells with DC661 results in a more striking accumulation of the autophagic vesicle marker LC3B-II at lower concentrations compared with either Lys05 or HCQ, reflecting more pronounced accumulation of autophagic vesicles at concentrations between 0.1 and 10 μmol/L. All cells die at concentrations above 10 μmol/L for DC661 in contrast to Lys05 and HCQ. Compared with HCQ or Lys05, DC661 treatment induces a significantly more potent inhibition of autophagic flux in melanoma cells expressing the mCherry-eGFP-LC3B reporter, and significantly higher levels of free GFP in melanoma cells expressing GFP-LC3B. DC661 treatment resulted in significantly greater lysosomal deacidification compared with either HCQ or Lys05. The IC50 of DC661 in 72-hour MTT assays is 100-fold lower than that of HCQ across multiple cancer cell lines including colon and pancreas cancer cell lines. DC661 suppresses long-term clonogenic growth of melanoma cells more effectively and induces significantly more apoptosis than Lys05, HCQ, or combined BRAF and MEK inhibition in BRAF-mutant melanoma cells[1].
In vivo

Treatment with the reduced dose (3 mg/kg, i.p.) of DC661 in a HT29 xenograft results in a significant reduction in tumor volume and almost complete suppression of daily tumor growth rate compared with control mice without significantly affecting mouse weight[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    A375P cells

  • Concentrations

    0.1, 0.3, 1, 3, 10 μM

  • Incubation Time

    6 hours

  • Method

    A375P cells are treated with doses of HCQ, Lys05, or DC661 for 6 hours before lysates are immunoblotted.
Animal Study:

[1]
  • Animal Models

    HT29 colorectal xenograft

  • Dosages

    3 mg/kg

  • Administration

    i.p.

Selleck's DC661 has been cited by 15 publications

Divergent effects of acute and chronic PPT1 inhibition in melanoma [ Autophagy, 2024, 1-13.] PubMed: 39265628
Lysosomal lipid peroxidation regulates tumor immunity [ J Clin Invest, 2023, 133(8)e164596] PubMed: 36795483
Lysosomal lipid peroxidation regulates tumor immunity [ J Clin Invest, 2023, 133(8)e164596] PubMed: 36795483
Targeting AKT induced Ferroptosis through FTO/YTHDF2-dependent GPX4 m6A methylation up-regulating and degradating in colorectal cancer [ Cell Death Discov, 2023, 9(1):457] PubMed: 38102129
ASCL2 Maintains Stemness Phenotype through ATG9B and Sensitizes Gliomas to Autophagy Inhibitor [ Adv Sci (Weinh), 2022, 9(27):e2105938] PubMed: 35882624
Calenduloside E protects against myocardial ischemia-reperfusion injury induced calcium overload by enhancing autophagy and inhibiting L-type Ca2+ channels through BAG3 [ Biomed Pharmacother, 2022, 145:112432] PubMed: 34798472
SYVN1-mediated ubiquitination and degradation of MSH3 promotes the apoptosis of lens epithelial cells [ FEBS J, 2022, 10.1111/febs.16447] PubMed: 35334159
Poly (ADP-ribose) polymerase 1-mediated defective mitophagy contributes to painful diabetic neuropathy in the db/db model [ J Neurochem, 2022, 10.1111/jnc.15606] PubMed: 35263449
ER proteins decipher the tubulin code to regulate organelle distribution [ Nature, 2021, 10.1038/s41586-021-04204-9] PubMed: 34912111
E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability [ Cell Death Dis, 2021, 12(3):239] PubMed: 33664240

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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