Dansylcadaverine (Monodansyl cadaverine)

Catalog No.E0451 Batch:E045101

Technical Data

Formula	C₁₇H₂₅N₃O₂S
Molecular Weight	335.46	CAS No.	10121-91-2
Solubility (25°C)*	In vitro	DMSO	67 mg/mL (199.72 mM)
		Ethanol	8 mg/mL (23.84 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Dansylcadaverine (Monodansyl cadaverine, MDC) is an autofluorescent compound used for the labeling of autophagic vacuoles. Dansylcadaverine, a high affinity substrate of transglutaminases, can block the receptor-mediated endocytosis of many ligands.
In vitro	Dansylcadaverine is an autofluorescent compound used for the labeling of autophagic vacuoles, which is able to serve as a substrate for transglutaminases and to block competitively the crosslinking of fibrin molecules.^[3]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines MCF7 cells Concentrations 50 mM Incubation Time 15 min Method MCF7 cells (2.4 ×10⁴ ) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-MA for different time periods. The cells are then incubated with 50 mM monodansylcadaverine at 37℃ for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope.

Cell Assay:^{^[3]}

Cell lines
MCF7 cells
Concentrations
50 mM
Incubation Time
15 min
Method
MCF7 cells (2.4 ×10⁴ ) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-MA for different time periods. The cells are then incubated with 50 mM monodansylcadaverine at 37℃ for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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