Dac51

Catalog No.S9876 Batch:S987602

Technical Data

Formula

C₂₀H₁₇Cl₂F₃N₄O₄

Molecular Weight

505.27

CAS No.

2243944-92-3 (free base)

Solubility (25°C)*

In vitro

DMSO

100 mg/mL (197.91 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 Corn oil	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Dac51 is a potent FTO inhibitor with an IC50 of 0.4 μM for inhibiting FTO demethylation.
In vitro	Dac51 can serve as a potent FTO inhibitor to dampen the glycolytic capacity of tumor cells by inhibiting FTO-mediated demethylation on transcripts including Jun and Cebpb.[1]
In vivo	Dac51, a FTO inhibitor, delivers strong T cell-mediated antitumor effects and prevents tumor recurrence via increasesing CD8+ T cell inﬁltration in tumors, in multiple vivo cancer models.[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines B16-OVA cells, LLC cell line, LLC-zsGreen-OTIp cells, MC38 cell line Concentrations 1 μM, 5 μM Incubation Time 48 h Method Cells are cultured in Dulbecco s modified Eagles medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, and 1% non-essential amino acids at 37 ℃ 5% CO2. Cells are cultured with Dac51 (1 mM, 5mM) for 48 hr.Then cell growth is monitored by Trypan Blue staining. Apoptosis is analyzed through ﬂow cytometry. Cell total RNA is extracted for m6A dot blot assay.
Animal Study:^{^[1]}	Animal Models C57BL/6 mice inoculated with Fto-Kd or B16-OVA tumor cells, Rag2/ mice inoculated with B16-OVA cells Dosages 2 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
B16-OVA cells, LLC cell line, LLC-zsGreen-OTIp cells, MC38 cell line
Concentrations
1 μM, 5 μM
Incubation Time
48 h
Method
Cells are cultured in Dulbecco s modified Eagles medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, and 1% non-essential amino acids at 37 ℃ 5% CO2. Cells are cultured with Dac51 (1 mM, 5mM) for 48 hr.Then cell growth is monitored by Trypan Blue staining. Apoptosis is analyzed through ﬂow cytometry. Cell total RNA is extracted for m6A dot blot assay.

Animal Study:^{^[1]}

Animal Models
C57BL/6 mice inoculated with Fto-Kd or B16-OVA tumor cells, Rag2/ mice inoculated with B16-OVA cells
Dosages
2 mg/kg
Administration
i.p.

References

[1] Yi Liu, et al. Cell Metab. 2021 Jun 1;33(6):1221-1233.e11.

Selleck's Dac51 has been cited by 1 publication

The Functional Role and Regulatory Mechanism of FTO m6A RNA Demethylase in Human Uterine Leiomyosarcoma [ Int J Mol Sci, 2023, 24(9)7957]	PubMed: 37175660

The Functional Role and Regulatory Mechanism of FTO m6A RNA Demethylase in Human Uterine Leiomyosarcoma [ Int J Mol Sci, 2023, 24(9)7957]

PubMed: 37175660

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.