D-Pinitol

Catalog No.S3870 Batch:S387001

Technical Data

Formula	C₇H₁₄O₆
Molecular Weight	194.18	CAS No.	10284-63-6
Solubility (25°C)*	In vitro	DMSO	38 mg/mL (195.69 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	D-pinitol (Methylinositol, Pinitol, 3-O-Methyl-D-chiro-inositol, D-(+)-Pinitol, Inzitol) is a naturally occurring compound derived from soy and has significant pharmacological activitites such as inhibition of the T-helpercell-1 response, antiviral, larvicidal, antiinflammatory, antihyperlipidemic, cardioprotective, inhibition of ovalbumin-induced airway inflammation and antioxidant.
In vitro	D-pinitol reduces the migration and the invasion of prostate cancer cells (PC3 and DU145) at noncytotoxic concentrations. Treatment of prostate cancer cells with D-pinitol reduces mRNA and cell surface expression of αvβ3 integrin. In addition, D-pinitol exerts its inhibitory effects by reducing focal adhesion kinase (FAK) phosphorylation, c-Src kinase activity and NF-κB activation^[1].
In vivo	D-pinitol exerts an acute and chronically-sustained antihyperglycaemic effect in the murine STZ-induced model of hypoinsulinaemic diabetes. The mechanism of action of D-pinitol does not augment the effect of insulin but might involve an interaction with part of a cellular signalling pathway that links insulin with glucose transport^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human prostate cancer cell lines (PC3 and DU145) Concentrations 0, 1, 3, 10 and 30 μM Incubation Time 24 h Method For invasion assay, filters are precoated with 30 μL Matrigel basement membrane matrix for 30 min. The following procedures are the same for both migration and invasion assays. After the treatment with D-pinitol (0, 1, 3, 10 and 30 μM) for 24 h, cells are harvested and seeded to Transwell at 1 × 10⁴ cells/well in serum-free medium and then incubated for 24 h at 37 °C in 5% CO2. Cells are then fixed in 3.7% formaldehyde for 5 min and stained with 0.05% crystal violet in PBS for 15 min. Cells on the upper side of the filters are removed with cotton-tipped swabs, and the filters are washed with PBS. Cells on the underside of the filters are examined and counted under a microscope.
Animal Study:^{^[2]}	Animal Models Obese-diabetic ob/ob mice Dosages up to 100 mg/kg Administration p.o./i.p.

Cell Assay:^{^[1]}

Cell lines
Human prostate cancer cell lines (PC3 and DU145)
Concentrations
0, 1, 3, 10 and 30 μM
Incubation Time
24 h
Method
For invasion assay, filters are precoated with 30 μL Matrigel basement membrane matrix for 30 min. The following procedures are the same for both migration and invasion assays. After the treatment with D-pinitol (0, 1, 3, 10 and 30 μM) for 24 h, cells are harvested and seeded to Transwell at 1 × 10⁴ cells/well in serum-free medium and then incubated for 24 h at 37 °C in 5% CO2. Cells are then fixed in 3.7% formaldehyde for 5 min and stained with 0.05% crystal violet in PBS for 15 min. Cells on the upper side of the filters are removed with cotton-tipped swabs, and the filters are washed with PBS. Cells on the underside of the filters are examined and counted under a microscope.

Animal Study:^{^[2]}

Animal Models
Obese-diabetic ob/ob mice
Dosages
up to 100 mg/kg
Administration
p.o./i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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