D-Luciferin sodium salt

Catalog No.S6924 Batch:S692403

Technical Data

Formula

C₁₁H₇N₂NaO₃S₂

Molecular Weight

302.3

CAS No.

103404-75-7

Solubility (25°C)*

In vitro

Water (warmed with 50ºC water bath)

50 mg/mL (165.39 mM)

DMSO

20 mg/mL (66.15 mM)

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	0.3mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	D-Luciferin (D-(-)-Luciferin, Firefly luciferin) sodium salt is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.
In vitro	1.Example protocol for in vitro bioluminescent image assays a) Prepare a 100 mM (100-200X) Luciferin stock solution in sterile water. Mix well. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light. b) Prepare a 0.5-1 mM working solution of D-Luciferin in pre-warmed tissue culture medium. c) Aspirate media from cultured cells. d) Add Luciferin working solution to cells, and incubate the cells for 5-10 minutes at 37℃ just prior to imaging. 2.Example protocol for in vivo bioluminescent image assays a) Prepare a 15 mg/mL Luciferin stock solution in DPBS, without Mg2+ and Ca2+. Mix well. b) Filter sterilizes the solution through a 0.2 μm filter. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light. c) Inject the luciferin intra-peritoneally (i.p.) 10-15 minutes before imaging at 150 mg/kg (or 10 μL/g of luciferin stock solution) of the animal body weight. Note: A kinetic study of luciferin should be performed for each animal model to determine peak signal time. 3.Example protocol for luciferin reporter assays a) Prepare a 100 mM Luciferin stock solution in sterile water. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light. b) Prepare a 1 mM working solution of D-Luciferin with 3 mM ATP, 1 mM DTT and 15 mM MgSO4 in 25 mM tricine buffer pH 7.8. c) Pipette 5-10 μLof cell lysate into a microplate. Use lysis reagent or buffer without lysate as a blank. d) Prime luminometer with luciferin working solution. e) Inject 200 μL of luciferin working solution with no delay and a 10 second integration time.

Description

D-Luciferin (D-(-)-Luciferin, Firefly luciferin) sodium salt is the natural substrate of luciferases that catalyze the production of light in bioluminescent insects.

In vitro

1.Example protocol for in vitro bioluminescent image assays
a) Prepare a 100 mM (100-200X) Luciferin stock solution in sterile water. Mix well. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light.
b) Prepare a 0.5-1 mM working solution of D-Luciferin in pre-warmed tissue culture medium.
c) Aspirate media from cultured cells.
d) Add Luciferin working solution to cells, and incubate the cells for 5-10 minutes at 37℃ just prior to imaging.
2.Example protocol for in vivo bioluminescent image assays
a) Prepare a 15 mg/mL Luciferin stock solution in DPBS, without Mg2+ and Ca2+. Mix well.
b) Filter sterilizes the solution through a 0.2 μm filter. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light.
c) Inject the luciferin intra-peritoneally (i.p.) 10-15 minutes before imaging at 150 mg/kg (or 10 μL/g of luciferin stock solution) of the animal body weight.
Note: A kinetic study of luciferin should be performed for each animal model to determine peak signal time.
3.Example protocol for luciferin reporter assays
a) Prepare a 100 mM Luciferin stock solution in sterile water. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light.
b) Prepare a 1 mM working solution of D-Luciferin with 3 mM ATP, 1 mM DTT and 15 mM MgSO4 in 25 mM tricine buffer pH 7.8.
c) Pipette 5-10 μLof cell lysate into a microplate. Use lysis reagent or buffer without lysate as a blank.
d) Prime luminometer with luciferin working solution.
e) Inject 200 μL of luciferin working solution with no delay and a 10 second integration time.

Protocol (from reference)

References

[1] Giuseppe Meroni, et al. Special Issue Reviews and Accounts. ARKIVOC 2009 (i) 265-288.

Selleck's D-Luciferin sodium salt has been cited by 6 publications

Tumor-intrinsic YTHDF1 drives immune evasion and resistance to immune checkpoint inhibitors via promoting MHC-I degradation [ Nat Commun, 2023, 14(1):265]	PubMed: 36650153
Carbenoxolone disodium suppresses the migration of gastric cancer by targeting HDAC6 [ Future Med Chem, 2023, 15(4):333-344]	PubMed: 36946221
A magneto-controlled biocatalytic cascade with a fluorescent output [ Org Biomol Chem, 2022, 20(9):1869-1873]	PubMed: 35156979
Leronlimab, a humanized monoclonal antibody to CCR5, blocks breast cancer cellular metastasis and enhances cell death induced by DNA damaging chemotherapy [ Breast Cancer Res, 2021, 23(1):11]	PubMed: 33485378
KDM4A-mediated histone demethylation of SLC7A11 inhibits cell ferroptosis in osteosarcoma [ Biochem Biophys Res Commun, 2021, 550:77-83]	PubMed: 33689883
Tumor treating fields increases membrane permeability in glioblastoma cells [ Cell Death Discov, 2018, 4:113]	PubMed: 30534421

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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