D-Aspartic acid

Catalog No.S5129 Batch:S512901

Technical Data

Formula	C₄H₇NO₄
Molecular Weight	133.10	CAS No.	1783-96-6
Solubility (25°C)*	In vitro	5%TFA	6.02 mg/mL (45.22 mM)
		DMSO	0.01 mg/mL (0.07 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	D-Aspartic acid (D-Asp, Aminosuccinic acid, asparagic acid, asparaginic acid) is an endogenous amino acid occurring in several tissues and cells of both invertebrates and vertebrates. It can regulate testosterone synthesis and may act on a stimulatory receptor (NMDA).
In vitro	D-aspartate (D-Asp) is an endogenous amino acid present in vertebrate tissues, with particularly high levels in the testis. In vitro studies have demonstrated that D-Asp up-regulates testosterone production in Leydig cells by enhancing expression of the steroidogenic acute regulatory protein. Besides testosterone release, D-Asp induced E2 synthesis through aromatase activation^[1].
In vivo	In vivo studies indicate that D-Asp indirectly stimulates spermatogenesis through the hypothalamic-pituitary-gonadal axis. The oral and/or intraperitoneal D-Asp administration to adult rats induced an increase of serum progesterone and LH as well as an increase of testis/serum testosterone levels, whereas 17β-estradiol (E2) levels did no change^[1]. D-aspartic acid has significant analgesic activity^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines GC-1 cells Concentrations 200 μM Incubation Time 30 min, 2 h, 4 h Method Cultures were carried on in the presence of D-Asp (200 μM; diluted in distilled H2O) in different time exposure of the amino acid (30 min, 2 h, 4 h). Control cultures were treated with vehicle alone. At the end of the incubation period, cells were rinsed in PBS, harvested by trypsinization, collected by centrifugation at 1,000g for 10 min and immediately frozen at 80°C for biochemical analyses.
Animal Study:^{^[2]}	Animal Models Inbred albino BALB/C mice Dosages 55, 115, and 230 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
GC-1 cells
Concentrations
200 μM
Incubation Time
30 min, 2 h, 4 h
Method
Cultures were carried on in the presence of D-Asp (200 μM; diluted in distilled H2O) in different time exposure of the amino acid (30 min, 2 h, 4 h). Control cultures were treated with vehicle alone. At the end of the incubation period, cells were rinsed in PBS, harvested by trypsinization, collected by centrifugation at 1,000g for 10 min and immediately frozen at 80°C for biochemical analyses.

Animal Study:^{^[2]}

Animal Models
Inbred albino BALB/C mice
Dosages
55, 115, and 230 mg/kg
Administration
i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.