Cyclic Pifithrin-α hydrobromide

Catalog No.S5791 Batch:S579101

Technical Data

Formula	C₁₆H₁₇BrN₂S
Molecular Weight	349.29	CAS No.	511296-88-1
Solubility (25°C)*	In vitro	DMSO	27 mg/mL (77.29 mM)
		Ethanol	3 mg/mL (8.58 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Cyclic Pifithrin-α hydrobromide is the hydrobromide salt form of Cyclic Pifithrin-α, which is a transcriptional inhibitor of p53.

Targets

p53 ^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines H460 and IGROV-1 cell lines Concentrations 10 μM and 20 μM Incubation Time 24 h Method H460 and IGROV-1 cell lines were treated with either PFT or paclitaxel alone or with their combination for 24 hours. After washing with PBS, the cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized with ice-cold methanol for 20 minutes at −20°C. After blocking with PBA (PBS containing 1% bovine serum albumin), anti-p53 antibody was incubated for 1 hour at room temperature followed by incubation with Alexa Fluor 488 goat antimouse IgG secondary antibody for 1 hour. Images were observed and analyzed by confocal microscopy.

Cell Assay:^{^[1]}

Cell lines
H460 and IGROV-1 cell lines
Concentrations
10 μM and 20 μM
Incubation Time
24 h
Method
H460 and IGROV-1 cell lines were treated with either PFT or paclitaxel alone or with their combination for 24 hours. After washing with PBS, the cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized with ice-cold methanol for 20 minutes at −20°C. After blocking with PBA (PBS containing 1% bovine serum albumin), anti-p53 antibody was incubated for 1 hour at room temperature followed by incubation with Alexa Fluor 488 goat antimouse IgG secondary antibody for 1 hour. Images were observed and analyzed by confocal microscopy.

References

[1] Zuco V, et al. Neoplasia. 2008, 10(6):587-96.

Selleck's Cyclic Pifithrin-α hydrobromide has been cited by 2 publications

A transcriptional program associated with cell cycle regulation predominates in the anti-inflammatory effects of CX-5461 in macrophage [ Front Pharmacol, 2022, 13:926317]	PubMed: 36386132
Oxidative stress induces monocyte-to-myofibroblast transdifferentiation through p38 in pancreatic ductal adenocarcinoma [ Clin Transl Med, 2020, 10.1002/ctm2.41]	PubMed: 32508052

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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