Cucurbitacin B

Catalog No.S8165 Batch:S816501

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Technical Data

Formula

C32H46O8
Molecular Weight 558.70 CAS No. 6199-67-3
Solubility (25°C)* In vitro DMSO 100 mg/mL (178.98 mM)
Ethanol 50 mg/mL (89.49 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Cucurbitacin B, an oxygenated tetracyclic triterpenoid compound extracted from Cucurbitaceae plant species, is a long-term anticancer agent by disruption of microtubule polymerization.
Targets
Microtubule [1]
In vitro Cucurbitacin B inhibits the growth of 5 GBM cell lines: U87, U118, U343, U373 and T98G by affecting the cytoskeleton and the JNK pathway, with ED50 ranged from 5 nM to 100 nM. Cucurbitacin B also causes cell cycle arrest at the G2/M phase, decreases cell migration and induces apoptosis of GBM cells. [1] In human breast cancer cell lines, Cucurbitacin B also inhibits cell growth with ED50 varied from 30.3 nM to 418 nM, and rapidly induces cytoskeletal disruption. [2] In addition, Cucurbitacin B possesses immunomodulatory and anti-inflammatory effects, depending on the induction of heme oxygenase-1 expression via Nrf2 activation. [3]
In vivo In nude mice bearing MDA-MB-231 tumors, Cucurbitacin B (1 mg/kg, i.p.) inhibits tumor growth by 55 %, and causes lower serum glucose levels. [2]

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    U87, T98G, U118, U343, and U373 cells

  • Concentrations

    ~1 μM

  • Incubation Time

    48 h

  • Method

    For proliferation measurements, the cells are placed into 96 well plates, and cell growth is measured at various times by MTT assay according to the protocol.

Selleck's Cucurbitacin B has been cited by 3 publications

NLRP3 inflammasome inhibitor cucurbitacin B suppresses gout arthritis in mice [ J Mol Endocrinol, 2021, 67(2):27-40] PubMed: 34047713
Targeting NAD+ biosynthesis overcomes panobinostat and bortezomib-induced malignant glioma resistance. [ Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0669] PubMed: 32238439
Cucurbitacin B inhibits cell proliferation and induces cell apoptosis in colorectal cancer by modulating methylation status of BTG3 [ Neoplasma, 2019, 66(4):593-602] PubMed: 31058532

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.