CU-CPT-8m

Catalog No.S4461 Batch:S446101

Technical Data

Formula	C₁₄H₁₂N₄O
Molecular Weight	252.27	CAS No.	125079-83-6
Solubility (25°C)*	In vitro	DMSO	6 mg/mL (23.78 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

CU-CPT-8m (CPD1578) is a specific antagonist of toll-like receptor 8(TLR8) with IC50 of 67 nM and Kd of 220 nM.

Targets

TLR8 ^[1] (Cell-free assay)	TLR8 ^[1] (Cell-free assay)
67 nM	220 nM(Ki)

In vitro

CU-CPT8m, recognizes a pocket on the interface of two copies of TLR8 and prevents them from undergoing conformational changes that are necessary for the proinflammatory signaling.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines HEK-Blue TLR8 cells Concentrations 24 h Incubation Time 0.1 nM-10 mM Method HEK-Blue TLR8 cells were seeded in tissue culture treated 96-well plates with a density of 7.5 × 10⁴ cells/well in DMEM media supplemented with 10% FBS (phosphatases deactivated with heat). These cells were then treated with R848 (1 μg/mL) along with various concentrations of appropriate compounds. Plates are then incubated in humidified incubators containing 5% CO2 at 37 °C for 24 h. Supernatant was removed, and 100 µL of 1:10 diluted WST-1 (Roche) was then added to each well. The plate was then incubated at 37 ℃ till color change (30 min-1 h).

Cell Assay:^{^[2]}

Cell lines
HEK-Blue TLR8 cells
Concentrations
24 h
Incubation Time
0.1 nM-10 mM
Method
HEK-Blue TLR8 cells were seeded in tissue culture treated 96-well plates with a density of 7.5 × 10⁴ cells/well in DMEM media supplemented with 10% FBS (phosphatases deactivated with heat). These cells were then treated with R848 (1 μg/mL) along with various concentrations of appropriate compounds. Plates are then incubated in humidified incubators containing 5% CO2 at 37 °C for 24 h. Supernatant was removed, and 100 µL of 1:10 diluted WST-1 (Roche) was then added to each well. The plate was then incubated at 37 ℃ till color change (30 min-1 h).

References

Selleck's CU-CPT-8m has been cited by 1 publication

Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia [ Nat Commun, 2024, 15(1):5678]	PubMed: 38971858

Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia [ Nat Commun, 2024, 15(1):5678]

PubMed: 38971858

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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