Cremophor EL

Technical Data

Biological Activity

Protocol (from reference)

References

Selleck's Cremophor EL has been cited by 1 publication

Catalog No.S6828 Batch:S682801

Formula

Molecular Weight

CAS No.

61791-12-6

Solubility (25°C)*

In vitro

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	1.25mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.25mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Cell Assay:^{^[1]}	Cell lines Human cell lines brain microvascular endothelial, Caco-2 cells Concentrations 0.1-50 mg/ml Incubation Time 24 h Method Cell lines are seeded in culture dishes at a density of 5 × 10₄ cells/cm₂, and the medium is changed every 2 days. Cremophor EL are tested at 0.1–50 mg/mL concentrations. Triton X-100 detergent (10 mg/mL) is used in toxicity assays as a reference compound to cause cell death.

Cell Assay:^{^[1]}

Cell lines
Human cell lines brain microvascular endothelial, Caco-2 cells
Concentrations
0.1-50 mg/ml
Incubation Time
24 h
Method
Cell lines are seeded in culture dishes at a density of 5 × 10₄ cells/cm₂, and the medium is changed every 2 days. Cremophor EL are tested at 0.1–50 mg/mL concentrations. Triton X-100 detergent (10 mg/mL) is used in toxicity assays as a reference compound to cause cell death.

Description

Cremophor EL, a polyethoxylated surfactant, is a formulation vehicle used for various poorly-water soluble drugs, including the anticancer agent paclitaxel (Taxol). Cremophor EL is used in severe anaphylactoid hypersensitivity reactions, hyperlipidaemia, abnormal lipoprotein patterns, aggregation of erythrocytes and peripheral neuropathy.

Cremophor EL is a widely used excipient. In the first 10 h, Cremophor EL at 5 mg/mL increases the impedance, then decreases it, and after 20 h of treatment, epithelial cell death is detected. Cremophor EL in concentrations corresponding to clinical doses causes endothelial and epithelial toxicity. Both epithelial viability and monolayer integrity are seriously impaired at 10 to 50 mg/mL concentrations of Cremophor EL at 24-h treatment. Cremophor EL also enhances cell detachment.^[1]

Cremophor EL-BB4 formulation-mediated photodynamic therapy (PDT) disseminated cancer in CT26-Luc mouse colon carcinoma model of intraperitoneal (IP) and significantly prolonged survival in mice when used along with white light.

1.05 g/mL at 20 °C

Growth hormone receptor antagonism downregulates ATP-binding cassette transporters contributing to improved drug efficacy against melanoma and hepatocarcinoma in vivo [ Front Oncol, 2022, 12:936145]	PubMed: 35865483

Growth hormone receptor antagonism downregulates ATP-binding cassette transporters contributing to improved drug efficacy against melanoma and hepatocarcinoma in vivo [ Front Oncol, 2022, 12:936145]

PubMed: 35865483

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.