Cordycepin

Catalog No.S3610 Batch:S361001

Technical Data

Formula	C₁₀H₁₃N₅O₃
Molecular Weight	251.24	CAS No.	73-03-0
Solubility (25°C)*	In vitro	DMSO	50 mg/mL (199.01 mM)
		Water	25 mg/mL (99.5 mM)
		Ethanol	1 mg/mL (3.98 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Cordycepin (3'-Deoxyadenosine) is an adenosine analogue, which is readily phosphorylated to its mono-, di-, and triphosphate intracellularly. It has a very potent anti-cancer, anti-oxidant and anti-inflammatory activities.
In vitro	Cordycepin increases interleukin (IL)-10 expression, decreased IL-2 expression and suppresses T lymphocyte activity. It also up-regulates IL-1beta, IL-6, IL-8 and TNF-alpha and suppresses phytohemagglutinin (PHA)-induced production of IL-2, IL-4, IL-5, IFN-gamma and IL-12^[1]. The structure of Cordycepin is very much similar with cellular nucleoside, adenosine and acts like a nucleoside analogue. Cordycepin lacks 3' hydroxyl group in its structure. It provokes RNA chain termination and interferes in mTOR signal transduction. At higher doses, Cordycepin inhibits cell attachment and reduces focal adhesion. under low nutritional stress, Cordycepin activates AMPK which blocks the activity of mTORC1 and mTORC2 complex. The inactivated mTORC2 complex cannot activate AKT 1 kinase fully, which in turn blocks mTOR signal transduction inhibiting translation and further cell proliferation and growth. Cordycepin also induces apoptosis by enhancing JNK and p38 kinase activity and increasing the protein expression of Bcl-2 pro-apoptotic molecules^[2]. Cordycepin has anti-tumor effect on mouse melanoma and lung carcinoma cells and human oral cancer cells^[3].
In vivo	Orally administered cordycepin inhibits melanoma cell growth in mice with no adverse effects^[4]. Oral cordycepin administration at dose of 10 mg/kg significantly improves Y-maze learning performance both in healthy and ischemic mice. However, cordycepin at dose of 5 mg/kg enhanced Y-maze learning only in ischemic mice but not healthy mice. Cordycepin significantly decreases the neuronal loss induced by ischemia in hippocampal CA1 and CA3 regions^[5].

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines MA-10 cells Concentrations 10 μM, 100 μM, 1 mM, 2 mM and 5 mM Incubation Time 24 h Method MA-10 cells (6 × 10⁵) are seeded in 6-cm Petri dish with 2 mL serum medium. After 70-80% confluence, cells are treated without or with 10 μM, 100 μM, 1 mM, 2 mM and 5 mM cordycepin for 24 h. Cell morphology is then observed and recorded under light microscopy. Apoptosis is characterized by the loss of cellular contact with the matrix and the appearance of plasma membrane blebbing.
Animal Study:^{^[4]}	Animal Models C57BL/6Cr mice Dosages 5 and 15 mg/kg Administration oral

Cell Assay:^{^[3]}

Cell lines
MA-10 cells
Concentrations
10 μM, 100 μM, 1 mM, 2 mM and 5 mM
Incubation Time
24 h
Method
MA-10 cells (6 × 10⁵) are seeded in 6-cm Petri dish with 2 mL serum medium. After 70-80% confluence, cells are treated without or with 10 μM, 100 μM, 1 mM, 2 mM and 5 mM cordycepin for 24 h. Cell morphology is then observed and recorded under light microscopy. Apoptosis is characterized by the loss of cellular contact with the matrix and the appearance of plasma membrane blebbing.

Animal Study:^{^[4]}

Animal Models
C57BL/6Cr mice
Dosages
5 and 15 mg/kg
Administration
oral

References

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Selleck's Cordycepin has been cited by 2 publications

The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors [ Nat Commun, 2022, 13(1):5703]	PubMed: 36171202
Cordycepin (3'-Deoxyadenosine) Suppresses Heat Shock Protein 90 Function and Targets Tumor Growth in an Adenosine Deaminase-Dependent Manner [ Cancers (Basel), 2022, 14(13)3122]	PubMed: 35804893

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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