CNO (Clozapine N-oxide)

Catalog No.S6887 Batch:S688704

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Technical Data

Formula

C18H19ClN4O
Molecular Weight 342.82 CAS No. 34233-69-7
Solubility (25°C)* In vitro DMSO 69 mg/mL (201.27 mM)
Water 69 mg/mL (201.27 mM)
Ethanol 9 mg/mL (26.25 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
3.4mg/ml Taking the 1 mL working solution as an example, add 50 μL 68 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CNO (Clozapine N-oxide) is a metabolite of Clozapine (GLXC-06516) and an agonist of human muscarinic designer receptors (Designer Receptors Exclusively Activated by Designer Drugs, DREADDs). Clozapine is a potent dopamine (DA) antagonist and a selective muscarinic M4 receptor agonist.Solutions are unstable and should be fresh-prepared.
Targets
DREADD [1] Dopamine receptor [2] muscarinic M4 receptor [1]
In vitro

CNO is a synthetic small molecule actuator for muscarinic acetylcholine DREADDs.
In vivo

Clozapine N-oxide (CNO), an agonist of human muscarinic designer receptor (DREADDs), is converted to clozapine and exerts clozapine-like behavioral effects in both mice and rats further emphasizes the need for appropriate control groups in studies employing DREADDs.[4]

Protocol (from reference)

Animal Study:

[4]
  • Animal Models

    standard laboratory rats and mice

  • Dosages

    1.0, 3.2, 10.0 mg/kg for rats; 1.25, 2.5, 5.0, 10.0, 20.0 mg/kg for mice

  • Administration

    i.p.

Selleck's CNO (Clozapine N-oxide) has been cited by 12 publications

Distinct eLPBChAT projections for methamphetamine withdrawal anxiety and primed reinstatement of conditioned place preference [ Theranostics, 2024, 14(7):2881-2896] PubMed: 38773977
Distinct eLPBChAT projections for methamphetamine withdrawal anxiety and primed reinstatement of conditioned place preference [ Theranostics, 2024, 14(7):2881-2896] PubMed: 38773977
Pyruvate dehydrogenase complex E1 subunit α crotonylation modulates cocaine-associated memory through hippocampal neuron activation [ Cell Rep, 2024, 43(8):114529] PubMed: 39046876
Projection neurons from medial entorhinal cortex to basolateral amygdala are critical for the retrieval of morphine withdrawal memory [ iScience, 2024, 27(7):110239] PubMed: 39021787
Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion [ Nat Commun, 2023, 10.1038/s41467-023-42814-1] PubMed: 37903803
Nucleus accumbens circuit disinhibits lateral hypothalamus glutamatergic neurons contributing to morphine withdrawal memory in male mice [ Nat Commun, 2023, 14(1):71] PubMed: 36604429
Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion [ Nat Commun, 2023, 14(1):6923] PubMed: 37903803
Tet2 acts in the lateral habenula to regulate social preference in mice [ Cell Rep, 2023, 42(7):112695] PubMed: 37402169
Tet2 acts in the lateral habenula to regulate social preference in mice [ Cell Rep, 2023, 42(7):112695] PubMed: 37402169
Excitatory neurons in the lateral parabrachial nucleus mediate the interruptive effect of inflammatory pain on a sustained attention task [ J Transl Med, 2023, 21(1):896] PubMed: 38072957

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.