Cholesterol

Catalog No.S4154 Batch:S415402

Technical Data

Formula

C₂₇H₄₆O

Molecular Weight

386.65

CAS No.

57-88-5

Solubility (25°C)*

In vitro

Ethanol

14 mg/mL (36.2 mM)

DMSO

Insoluble

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%ethyl alcohol 1 40%PEG300 2 5%Tween80 3 50%ddH2O	0.7mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clarified ethyl alcohol stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% ethyl alcohol 1 95% Corn oil	0.7mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clear ethyl alcohol stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Cholesterol(Cholesterin) is the principal sterol of all higher animals required to build and maintain membranes.
In vitro	Cholesterol serves as a precursor for the biosynthesis of steroid hormones, bile acids, and vitamin D. It modulates membrane fluidity over the range of physiological temperatures. The hydroxyl group on cholesterol interacts with the polar head groups of the membrane phospholipids and sphingolipids, while the bulky steroid and the hydrocarbon chain are embedded in the membrane, alongside the nonpolar fatty-acid chain of the other lipids. Through the interaction with the phospholipid fatty-acid chains, cholesterol increases membrane packing, which reduces membrane fluidity. The structure of the tetracyclic ring of cholesterol contributes to the decreased fluidity of the cell membrane as the molecule is in a trans conformation making all but the side chain of cholesterol rigid and planar. In this structural role, cholesterol reduces the permeability of the plasma membrane to neutral solutes, protons, and sodium ions.
In vivo	Cholesterol is recycled. The liver excretes it in a non-esterified form (via bile) into the digestive tract. Typically about 50% of the excreted cholesterol is reabsorbed by the small bowel back into the bloodstream.

Protocol (from reference)

Selleck's Cholesterol has been cited by 11 publications

Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration [ Clin Transl Med, 2023, 13(9):e1415]	PubMed: 37740460
Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration [ Clin Transl Med, 2023, 13(9):e1415]	PubMed: 37740460
Screening of Natural Compounds for CYP11A1 Stimulation Against Cell Renal Cell Carcinoma [ Biol Proced Online, 2023, 25(1):31]	PubMed: 38036976
Vitamin D3 suppresses the cholesterol homeostasis pathway in patient-derived glioma cell lines [ FEBS Open Bio, 2023, 13(9):1789-1806]	PubMed: 37489660
Vitamin D3 suppresses the cholesterol homeostasis pathway in patient-derived glioma cell lines [ FEBS Open Bio, 2023, 13(9):1789-1806]	PubMed: 37489660
Copy number amplification of ENSA promotes the progression of triple-negative breast cancer via cholesterol biosynthesis [ Nat Commun, 2022, 13(1):791]	PubMed: 35145111
Inhibition of NPC1L1 disrupts adaptive responses of drug-tolerant persister cells to chemotherapy [ EMBO Mol Med, 2022, 14(2):e14903]	PubMed: 35023619
The lipid transporter HDLBP promotes hepatocellular carcinoma metastasis through BRAF-dependent epithelial-mesenchymal transition [ Cancer Lett, 2022, 549:215921]	PubMed: 36122630
p53 transcriptionally regulates SQLE to repress cholesterol synthesis and tumor growth [ EMBO Rep, 2021, e52537]	PubMed: 34459531
Pitavastatin stimulates retinal angiogenesis via HMG-CoA reductase-independent activation of RhoA-mediated pathways and focal adhesion [ Graefes Arch Clin Exp Ophthalmol, 2021, 10.1007/s00417-021-05328-4]	PubMed: 34328550

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.