Chlorpromazine

Catalog No.S5749 Batch:S574904

Technical Data

Formula	C₁₇H₁₉ClN₂S
Molecular Weight	318.86	CAS No.	50-53-3
Solubility (25°C)*	In vitro	DMSO	65 mg/mL (203.85 mM)
		Ethanol	64 mg/mL (200.71 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Chlorpromazine (CPZ) is a low-potency typical antipsychotic agent that is used to treat psychotic disorders such as schizophrenia. Chlorpromazine (CPZ) inhibits 5-HT(2A) receptor and dopamine D2 in vitro. Chlorpromazine (CPZ) also inhibits sodium channel and blocks HERG potassium channels with IC50 of 21.6 μM.
In vitro	Chlorpromazine exerts a mild decrease in sEV uptake in only one MM cell line, indicating that the uptake of BMSC-derived sEVs by suspension MM cells may not rely on chlorpromazine-targeted receptors.^[1]
In vivo	Chlorpromazine suppresses the growth of endometrial cancer (EC) tumors in vivo at a low dose with no signs of toxicity.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MM1S cells, RPMI 8226, U266, H929 cells Concentrations 5, 10, 20 μM Incubation Time 30 min for MM1S cells, 4.5 h for RPMI 8226, U266, H929 cells Method MM1S cells pre-treated with heparin, cytochalasin D (Cyto D), dynasore, genistein, or chlorpromazine (CPZ) at the indicated concentrations for 30 min are cultured with 50 μg/mL DID-labeled BMSC-derived sEVs for another 4 h. The mean fluorescence intensity of DID in these cells is determined using flow cytometry. RPMI 8226, U266, or H929 cells are treated with heparin, Cyto D, dynasore, genistein, or CPZ at the indicated concentration for 4.5 h in the absence of sEVs and their cell viability is measured.
Animal Study:^{^[2]}	Animal Models female BALB/c nude mice Dosages 3 or 12 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MM1S cells, RPMI 8226, U266, H929 cells
Concentrations
5, 10, 20 μM
Incubation Time
30 min for MM1S cells, 4.5 h for RPMI 8226, U266, H929 cells
Method
MM1S cells pre-treated with heparin, cytochalasin D (Cyto D), dynasore, genistein, or chlorpromazine (CPZ) at the indicated concentrations for 30 min are cultured with 50 μg/mL DID-labeled BMSC-derived sEVs for another 4 h. The mean fluorescence intensity of DID in these cells is determined using flow cytometry. RPMI 8226, U266, or H929 cells are treated with heparin, Cyto D, dynasore, genistein, or CPZ at the indicated concentration for 4.5 h in the absence of sEVs and their cell viability is measured.

Animal Study:^{^[2]}

Animal Models
female BALB/c nude mice
Dosages
3 or 12 mg/kg
Administration
i.p.

References

Selleck's Chlorpromazine has been cited by 39 publications

Gonococcal OMV-delivered PorB induces epithelial cell mitophagy [ Nat Commun, 2024, 15(1):1669]	PubMed: 38396029
Proton pump inhibitors enhance macropinocytosis-mediated extracellular vesicle endocytosis by inducing membrane v-ATPase assembly [ J Extracell Vesicles, 2024, 13(4):e12426]	PubMed: 38532609
Proton pump inhibitors enhance macropinocytosis-mediated extracellular vesicle endocytosis by inducing membrane v-ATPase assembly [ J Extracell Vesicles, 2024, 13(4):e12426]	PubMed: 38532609
Extracellular Vesicle Spherical Nucleic Acids [ JACS Au, 2024, 4(6):2381-2392]	PubMed: 38938802
The combination therapy using tyrosine kinase receptors inhibitors and repurposed drugs to target patient-derived glioblastoma stem cells [ Biomed Pharmacother, 2024, 176:116892]	PubMed: 38876048
Protection of HT22 neuronal cells against chemically-induced ferroptosis by catechol estrogens: protein disulfide isomerase as a mechanistic target [ Sci Rep, 2024, 14(1):23988]	PubMed: 39402104
Real-Time Dissection of the Transportation and miRNA-Release Dynamics of Small Extracellular Vesicles [ Adv Sci (Weinh), 2023, 10(7):e2205566]	PubMed: 36599707
Treatment of Ulcerative Colitis by Cationic Liposome Delivered NLRP3 siRNA [ Int J Nanomedicine, 2023, 18:4647-4662]	PubMed: 37605735
Porcine deltacoronavirus enters ST cells by clathrin-mediated endocytosis and does not require Rab5, Rab7, or Rab11 [ Microbiol Spectr, 2023, 10.1128/spectrum.02553-23]	PubMed: 37962380
Evaluation of the liver targeting and anti‑liver cancer activity of artesunate‑loaded and glycyrrhetinic acid‑coated nanoparticles [ Exp Ther Med, 2023, 26(5):516]	PubMed: 37854499

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SHIPPING AND STORAGE
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