Cemiplimab (anti-PD-1)

Catalog No.A2022        Batch: A202202

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Technical Data

CAS No. 1801342-60-8
Formulation PBS buffer. Contains no stabilizers or preservatives
Isotype Human IgG4
Source HEK293
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99%
Protein concentration 4.3 mg/ml
Endotoxin Level <1 EU/mg

Biological Activity

Description Cemiplimab (anti-PD-1) is an intravenous human monoclonal antibody directed against programmed cell death-1 receptor (PD-1) and blocks its interaction with programmed death ligands 1 (PD-L1) and 2 (PD-L2). Cemiplimab blocks T-cell inactivation and enhances the immune system's anti-tumor response.
In vitro

In cell-based assays, REGN2810 reverses PD-1-dependent attenuation of T-cell receptor signaling in engineered T cells and enhances responses of human primary T cells.[1]
In vivo

Cemiplimab (REGN2810) binds the humanized PD-1 receptor and inhibits growth of MC38 murine tumors in human PD-1 knock-in mice. As REGN2810 binds to cynomolgus monkey PD-1 with high affinity, pharmacokinetic and toxicologic assessment of REGN2810 is performed in cynomolgus monkeys. High doses of REGN2810 are well tolerated, without adverse immune-related effects.[1]

Protocol (Only for Reference)

Cell Assay:

  • Objective: T cell activation bioassays
    Cells: Jurkat/AP-1-Luc/hPD-1 cells, HEK293/hCD20 cells, HEK293/hCD20/hPD-L1 cells
    Concentrations: 0.1 pM-0.1 μM
    Incubation Time: 4-6 h
    Method: Antigenpresenting cell (APC)-like HEK293 cells are generated by lentiviral transduction of human CD20 and human PD-L1. T-cell receptor (TCR) activation is achieved by an antiCD3 x anti-CD20 bispecific antibody. To generate a dose response curve for anti-CD3 x anti-CD20, the bispecific molecule is serially diluted and tested with 50,000/well Jurkat/AP-1-Luc/hPD-1 and 10,000/well HEK293/hCD20 or HEK293/hCD20/hPD-L1 cells in a 96-well plate. Serially diluted REGN2810 is tested under similar conditions in the presence of a fixed concentration of anti-CD3 x anti-CD20 (100 pM). Plates are incubated at 37°C for 4-6 hours.
    Reference: https://pubmed.ncbi.nlm.nih.gov/28265006/

    Objective: T cell activation bioassays
    Cells: HEK293/hCD20/hPD-L1 cells, CD4+ T cells, HEK293/hCD20/hPD-L1 cells
    Concentrations: 0.1 pM-0.1 μM
    Incubation Time: 72 h
    Method: HEK293/hCD20/hPD-L1 cells are treated with 50 mg/mL mitomycin C for 30 minutes at 37°C to inhibit proliferation. Serially diluted REGN2810 is incubated with 50,000/well preactivated CD4+ T cells and 25,000/well of HEK293/hCD20/hPD-L1 cells in the presence of 2 nM anti-CD3 x anti-CD20 bispecific antibody in a 96-well plate for 72 hours.
    Reference: https://pubmed.ncbi.nlm.nih.gov/28265006/

    Cemiplimab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)
Animal Study:

  • Objective: MC38 mouse colon carcinoma
    Animal Models: adult human PD-1 knock-in mice
    Formulation: --
    Dosages: 0.3 mg/kg, 1 mg/kg, 3 mg/kg, 10 mg/kg
    Administration: i.p.
    Reference: https://pubmed.ncbi.nlm.nih.gov/28265006

    Objective: pharmacokinetic (PK) study
    Animal Models: cynomolgus monkeys (Macaca fascicularis)
    Formulation: --
    Dosages: 1 mg/kg, 5 mg/kg, 15 mg/kg
    Administration: i.v.
    Reference: https://pubmed.ncbi.nlm.nih.gov/28265006

    Cemiplimab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

Selleck's Cemiplimab (anti-PD-1) has been cited by 1 publication

Variable PD-1 glycosylation modulates the activity of immune checkpoint inhibitors [ Life Sci Alliance, 2024, 7(3)e202302368] PubMed: 38176728

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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