Cefradine

Catalog No.S4671 Batch:S467101

Technical Data

Formula	C₁₆H₁₉N₃O₄S
Molecular Weight	349.4	CAS No.	38821-53-3
Solubility (25°C)*	In vitro	Water	10 mg/mL (28.62 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Cefradine (Cephradine, Sefril, Anspor, Velosef) is a semi-synthetic cephalosporin antibiotic.
In vivo	Cephradine has a low order of acute, subacute, and chronic oral toxicity, and acute and subacute parenteral toxicity. After daily intravenous doses of cephradine has been given to monkeys for 2 weeks, these animals show no signs of nephrotoxicity. Cephradine does not induce any teratogenic changes in the offspring of either mice or rats. The route of administration has been a determinant in the choice of a cephalosporin antibiotic. It is a distinct therapeutic advantage that a single cephalosporin antibiotic, cephradine, may be used in both oral and parenteral preparations^[2].

Description

Cefradine (Cephradine, Sefril, Anspor, Velosef) is a semi-synthetic cephalosporin antibiotic.

In vivo

Cephradine has a low order of acute, subacute, and chronic oral toxicity, and acute and subacute parenteral toxicity. After daily intravenous doses of cephradine has been given to monkeys for 2 weeks, these animals show no signs of nephrotoxicity. Cephradine does not induce any teratogenic changes in the offspring of either mice or rats. The route of administration has been a determinant in the choice of a cephalosporin antibiotic. It is a distinct therapeutic advantage that a single cephalosporin antibiotic, cephradine, may be used in both oral and parenteral preparations^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines CHO/hPEPT1 cells Concentrations 1 mg/mL Incubation Time 10, 20, 30, 45, 60, 90 min Method Cells Are seeded in six-well culture plates (9.6 cm², growth area) at a density of 10⁵ cells/cm². At 2 days postseeding, the medium is removed and cells are washed twice with pH 6.0 uptake buffer. Independent studies are performed for each drug solution at 1 mg/mL made in pH 6.0 uptake buffer. At each time point (10, 20, 30, 45, 60, 90 min), drug solution is removed and cells are washed three times with ice-cold pH 6.0 uptake buffer. One milliliter of Milli-Q water is added to each well and incubated for 30 min at 25 °C. Cells are harvested and sonicated for 1-2 min. ZnSO₄ solution (8%, 200 mL) is added to the cell lysate, vortexed rigorously, and centrifuged for 5 min at 3000 rpm. After filtration of the supernatant through a membrane filter (0.45 mm), samples are analyzed by HPLC.
Animal Study:^{^[2]}	Animal Models Charles River CD Sprague-Dawley rats Dosages 100, 300, or 1,000 mg/kg Administration p.o.

Cell Assay:^{^[1]}

Cell lines
CHO/hPEPT1 cells
Concentrations
1 mg/mL
Incubation Time
10, 20, 30, 45, 60, 90 min
Method
Cells Are seeded in six-well culture plates (9.6 cm², growth area) at a density of 10⁵ cells/cm². At 2 days postseeding, the medium is removed and cells are washed twice with pH 6.0 uptake buffer. Independent studies are performed for each drug solution at 1 mg/mL made in pH 6.0 uptake buffer. At each time point (10, 20, 30, 45, 60, 90 min), drug solution is removed and cells are washed three times with ice-cold pH 6.0 uptake buffer. One milliliter of Milli-Q water is added to each well and incubated for 30 min at 25 °C. Cells are harvested and sonicated for 1-2 min. ZnSO₄ solution (8%, 200 mL) is added to the cell lysate, vortexed rigorously, and centrifuged for 5 min at 3000 rpm. After filtration of the supernatant through a membrane filter (0.45 mm), samples are analyzed by HPLC.

Animal Study:^{^[2]}

Animal Models
Charles River CD Sprague-Dawley rats
Dosages
100, 300, or 1,000 mg/kg
Administration
p.o.

References

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SHIPPING AND STORAGE
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