CDN1163

Catalog No.S6815 Batch:S681501

Technical Data

Formula

C₂₀H₂₀N₂O₂

Molecular Weight

320.39

CAS No.

892711-75-0

Solubility (25°C)*

In vitro

DMSO

64 mg/mL (199.75 mM)

Ethanol

64 mg/mL (199.75 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	5.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.5mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

CDN1163 is a small molecular allosteric activator of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) that improves Ca2+ homeostasis.

Targets

SERCA ^[1]

In vitro

CDN1163 treatment significantly reduces the hepatic expression of genes involved in gluconeogenesis and lipogenesis, attenuates ER stress response and ER stress-induced apoptosis, and improves mitochondrial biogenesis, possibly through SERCA2-mediated activation of AMP-activated protein kinase pathway.^[1]

In vivo

CDN1163 reduces blood glucose levels and improves metabolic parameters and gluconeogenic gene expression, reverses hepatic steatosis, inhibits ER stress and ER stress-induced apoptosis, and improves mitochondrial efficiency in ob/ob mice in vivo.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HEK cells Concentrations 10 μm Incubation Time 2 h Method Cells are grown in 96-well plates (n = 6) and exposed to CDN1163 (10 μm) or vehicle (DMSO) for 2 h followed by the addition of 200 μm H²O² compared with untreated controls. H²O² activates the unfolded protein response and is an inducer of ER stress-promoted apoptosis. After 16 h of incubation, cell viability is measured using Luminescent Cell Viability Assay.

Cell Assay:^{^[1]}

Cell lines
HEK cells
Concentrations
10 μm
Incubation Time
2 h
Method
Cells are grown in 96-well plates (n = 6) and exposed to CDN1163 (10 μm) or vehicle (DMSO) for 2 h followed by the addition of 200 μm H²O² compared with untreated controls. H²O² activates the unfolded protein response and is an inducer of ER stress-promoted apoptosis. After 16 h of incubation, cell viability is measured using Luminescent Cell Viability Assay.

References

[1] Kang S, et al. J Biol Chem. 2016 Mar 4;291(10):5185-98.

Selleck's CDN1163 has been cited by 3 publications

Proteome-wide CETSA reveals diverse apoptosis-inducing mechanisms converging on an initial apoptosis effector stage at the nuclear periphery [ Cell Rep, 2024, 43(10):114784]	PubMed: 39365699
Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice [ J Clin Invest, 2022, 132(8)e149160]	PubMed: 35426376
Restoration of Sarco/Endoplasmic Reticulum Ca2+-ATPase Activity Functions as a Pivotal Therapeutic Target of Anti-Glutamate-Induced Excitotoxicity to Attenuate Endoplasmic Reticulum Ca2+ Depletion [ Front Pharmacol, 2022, 13:877175]	PubMed: 35517826

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.