Catalpol

Catalog No.S3909 Batch:S390903

Technical Data

Formula

C₁₅H₂₂O₁₀

Molecular Weight

362.33

CAS No.

2415-24-9

Solubility (25°C)*

In vitro

DMSO

72 mg/mL (198.71 mM)

Water

72 mg/mL (198.71 mM)

Ethanol

4 mg/mL (11.03 mM)

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.6mg/ml	Taking the 1 mL working solution as an example, add 50 μL of clarified DMSO stock solution of 72 mg/ml to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% corn oil	3.6mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 72 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Catalpol (Catalpinoside, Digitalis purpurea L), an iridoid glucoside contained richly in the roots of the small flowering plant species Rehmannia glutinosa Libosch, has been shown to have antioxidation, anti-inflammation, anti-apoptosis and other neuroprotective properties and plays a role in neuroprotection against hypoxic/ischemic injury, AD and PD in both in vivo and in vitro models.
In vitro	Catalpol is able to inhibit HCT116 cell proliferation. Furthermore, catalpol induces apoptosis in HCT116 cells, which depends on the increased activities of caspase-3 and −9. In addition, catalpol reduces the expression of PI3K, p-Akt and Akt in HCT116 cells^[1]. In vitro, catalpol inhibits TNF-α, IL-6, IL-4 and IL-1β production and up-regulates IL-10 expression in LPS-stimulated alveolar macrophages^[2].
In vivo	Catalpol decreases peroxynitrite formation and consequently exerts cardioprotective effects through the PI3K/Akt signaling pathway in ischemic/reperfusion rats. Catalpol inhibits lung W/D ratio, myeloperoxidase activity of lung samples, the amounts of inflammatory cells and TNF-α, IL-6, IL-4 and IL-1β in BALF induced by LPS. The production of IL-10 in BALF is up-regulated by catalpol^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HCT116 cells Concentrations 0, 25, 50 and 100 µg/ml Incubation Time 24, 48, 72 h Method HCT116 cells (2×10⁴ cells/well) are seeded in 96-well plates and cell viability is detected using MTT. HCT116 cells are cultured with various concentrations of catalpol (0, 25, 50 and 100 µg/ml). Following treatment for 24, 48, and 72 h, 20 µl MTT solution (0.5 mg/ml) is added into each well and cells are incubated at 37°C for 4 h. The culture medium of each well is subsequently removed and 150 µl dimethyl sulfoxide is added into each well at room temperature whilst being shaken for 20 min. Absorbance is measured at 570 nm using a Bio-Rad ELISA reader.
Animal Study:^{^[2]}	Animal Models Mice acute lung injury model (Male BALB/c mice) Dosages 2.5, 5 and 10 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
HCT116 cells
Concentrations
0, 25, 50 and 100 µg/ml
Incubation Time
24, 48, 72 h
Method
HCT116 cells (2×10⁴ cells/well) are seeded in 96-well plates and cell viability is detected using MTT. HCT116 cells are cultured with various concentrations of catalpol (0, 25, 50 and 100 µg/ml). Following treatment for 24, 48, and 72 h, 20 µl MTT solution (0.5 mg/ml) is added into each well and cells are incubated at 37°C for 4 h. The culture medium of each well is subsequently removed and 150 µl dimethyl sulfoxide is added into each well at room temperature whilst being shaken for 20 min. Absorbance is measured at 570 nm using a Bio-Rad ELISA reader.

Animal Study:^{^[2]}

Animal Models
Mice acute lung injury model (Male BALB/c mice)
Dosages
2.5, 5 and 10 mg/kg
Administration
i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.