Carbendazim

Catalog No.S3676 Batch:S367601

Technical Data

Formula	C₉H₉N₃O₂
Molecular Weight	191.19	CAS No.	10605-21-7
Solubility (25°C)*	In vitro	DMSO	17 mg/mL (88.91 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Carbendazim (Mercarzole, Carbendazole) is a broad-spectrum systemic antimycotic and can be used to control a broad range of diseases on field crops, fruits, and vegetables, including sclerotinia rot of canola, wheat head blight, peanut leaf spot, and SB on rice. Its mode of action is to inhibit the formation of mitotic microtubules in of fungi.
In vitro	Carbendazim (methyl 2-benzimidazolecarbamate) is widely used as a systemic fungicide in human food production and appears to act on fungal tubulin. However, it also inhibits proliferation of human cancer cells, including drug- and multidrug-resistant and p53-deficient cell lines, including murine melanoma, human breast, ovarian, lung, leukemia, and colon carcinoma cell lines, arresting the cells at the G2/M phase of the cell cycle and inducing apoptosis. It inhibits the proliferation of MCF7 human breast cancer cells with IC50 of 10 μM and arrests mitosis at a similar concentration (8 μM), in concert with suppression of microtubule dynamic instability without appreciable microtubule depolymerization. With microtubules assembled in vitro from pure tubulin, carbendazim also suppresses dynamic instability, reducing the dynamicity by 50% at 10 μM, with only minimal (21%) reduction of polymer mass. Carbendazim binds to mammalian tubulin (Kd, 42.8 ± 4.0 μM)^[1].
In vivo	Carbendazim (CBZ) is active in vivo against human pancreatic, lung, prostate, colon, and breast tumor xenografts. Oral administrations with CBZ at 100 and 500 mg/kg body weight for 28 days induces hepatic lipid metabolism disorder which is characterized by significant increases of hepatic lipid accumulation and triglyceride (TG) levels in mice. The serum cholesterol (TC), high-density lipoprotein, and low-density lipoprotein levels also increase after CBZ exposure. CBZ may also induce the occuring of inflammation characterized by the increase of serum IL-1β and IL-6 levels^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Human MCF7 breast cancer cells Concentrations 0.5–50 μM Incubation Time 24 h Method MCF7/GFP cells seeded at 3 × 10⁴ cells/ml in six-well plates are allowed to adhere (24 h), after which the medium is replaced with medium containing carbendazim. Cells are incubated with a range of carbendazim concentrations (0.5-50 μM) for 24 h, rinsed with Versene (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8.1 mM Na2HPO4, 0.5 mM EDTA, pH 7.2), and detached with trypsin in Versene. Both adherent and floating cells are collected. For determination of cell proliferation, cells are stained with trypan blue and counted by hemacytometer.
Animal Study:^{^[2]}	Animal Models ICR mice Dosages 100 and 500 mg/kg Administration oral

Cell Assay:^{^[1]}

Cell lines
Human MCF7 breast cancer cells
Concentrations
0.5–50 μM
Incubation Time
24 h
Method
MCF7/GFP cells seeded at 3 × 10⁴ cells/ml in six-well plates are allowed to adhere (24 h), after which the medium is replaced with medium containing carbendazim. Cells are incubated with a range of carbendazim concentrations (0.5-50 μM) for 24 h, rinsed with Versene (137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8.1 mM Na2HPO4, 0.5 mM EDTA, pH 7.2), and detached with trypsin in Versene. Both adherent and floating cells are collected. For determination of cell proliferation, cells are stained with trypan blue and counted by hemacytometer.

Animal Study:^{^[2]}

Animal Models
ICR mice
Dosages
100 and 500 mg/kg
Administration
oral

References

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.