Calpeptin

Catalog No.S7396 Batch:S739601

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Technical Data

Formula

C20H30N2O4

Molecular Weight 362.46 CAS No. 117591-20-5
Solubility (25°C)* In vitro DMSO 72 mg/mL (198.64 mM)
Ethanol 72 mg/mL (198.64 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Calpeptin is a potent, cell-permeable calpain inhibitor with ID50 of 52 nM, 34 nM, 138 nM, and 40 nM for Calpain I (porcine erythrocytes), Calpain II (porcine kidney), Papainb, and Calpain I (human platelets), respectively. Calpeptin attenuates apoptosis and intracellular inflammatory changes in muscle cells.
Targets
Calpain II (porcine kidney) [1] Calpain I (human platelets) [1] Calpain I (porcine erythrocytes) [1] Papainb [1]
34 nM(ID50) 40 nM(ID50) 52 nM(ID50) 138 nM(ID50)
In vitro Calpeptin inhibits 20K phosphorylation in a dose-related manner in platelets stimulated by thrombin, ionomycin or collagen. [1] In differentiating PC12 cells, Calpeptin promotes neurite elongation via inhibition of Calpain activity. [2] In rat retinal ganglion cells, Calpeptin attenuates apoptosis, maintains normal whole-cell membrane potential, and thus provides functional neuroprotection. [3]
In vivo In a feline right ventricular (RV) PO (RVPO) model, calpeptin (0.6 mg/kg, i.v.) blocks the activation of calpain and caspase-3, cleavage of their substrates, and cardiomyocyte programmed cell death. [4] In a rat focal cerebral ischemia–reperfusion injury model, Calpeptin reduces the neuronal apoptosis in hippocampal CA1 sector via inhibition of the expression of Caspase-3. [5]

Protocol (from reference)

Animal Study:[4]
  • Animal Models

    Feline right ventricular (RV) PO (RVPO) model

  • Dosages

    0.6 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Data independently produced by , , Oncol Rep, 2017, 37(6):3651-3659]

Selleck's Calpeptin has been cited by 26 publications

Genetic and Functional Analyses of Patients with Marked Hypo-High-Density Lipoprotein Cholesterolemia [ J Atheroscler Thromb, 2024, 10.5551/jat.64579] PubMed: 38538338
Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling [ Proc Natl Acad Sci U S A, 2023, 120(43):e2304288120] PubMed: 37844244
Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling [ Proc Natl Acad Sci U S A, 2023, 120(43):e2304288120] PubMed: 37844244
The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential [ EMBO Rep, 2023, e55069.] PubMed: 36744297
The lactate-NAD+ axis activates cancer-associated fibroblasts by downregulating p62 [ Cell Rep, 2022, 39(6):110792] PubMed: 35545049
Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis [ Oxid Med Cell Longev, 2022, 2022:2566917] PubMed: 35498131
Calpain-1: a Novel Antiviral Host Factor Identified in Porcine Small Intestinal Mucus [ mBio, 2022, 13(5):e0035822] PubMed: 36102516
Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy [ Comput Struct Biotechnol J, 2022, 20:2442-2454] PubMed: 35602976
Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis [ Cell Death Discov, 2021, 7(1):374] PubMed: 34864819
Xanthohumol Is a Potent Pan-Inhibitor of Coronaviruses Targeting Main Protease [ Int J Mol Sci, 2021, 22(22)12134] PubMed: 34830015

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.