Calcium folinate (Leucovorin calcium)

Catalog No.S5136 Batch:S513601

Technical Data

Formula	C₂₀H₂₁N₇O₇.Ca
Molecular Weight	511.5	CAS No.	1492-18-8
Solubility (25°C)*	In vitro	Water	100 mg/mL (195.5 mM)
		DMSO	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Calcium folinate (Leucovorin calcium) is a metabolically active, reduced form of folic acid that is rapidly absorbed and extensively metabolized in the liver to other folic acid derivatives.Solutions are unstable and should be fresh-prepared.
In vivo	Calcium folinate (also known as Leucovorin) treatment has no effect on tumor growth. The combination of bortezomib and leucovorin is able to induce apoptosis and attenuate tumor growth in vivo^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF-7 cells Concentrations 0-100 μM Incubation Time 10 days Method The breast cells are grown in RPM11640 medium supplemented with 10% dialyzed fetal calf serum and 2 mM glutamine at an initial plating density of 500-1000 cells/plate. After 12 hr, the cells are treated for a 24-hr period with various MTX concentrations: 0.5, 1, 3, 10 μM. At the end of the 24-hr MTX exposure, the cells are washed two times with phosphate-buffered saline and placed in drug-free medium containing various concentrations of leucovorin. Colonies are allowed to grow for a lo-day period prior to staining and counting. Cloning efficiency is determined by comparing the number of colonies formed in MTX-treated cells to those of a control without MTX, but with exposure the corresponding leucovorin concentration.
Animal Study:^{^[2]}	Animal Models Human colorectal HCT116 xenograft tumors (female NSG mice) Dosages 80 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MCF-7 cells
Concentrations
0-100 μM
Incubation Time
10 days
Method
The breast cells are grown in RPM11640 medium supplemented with 10% dialyzed fetal calf serum and 2 mM glutamine at an initial plating density of 500-1000 cells/plate. After 12 hr, the cells are treated for a 24-hr period with various MTX concentrations: 0.5, 1, 3, 10 μM. At the end of the 24-hr MTX exposure, the cells are washed two times with phosphate-buffered saline and placed in drug-free medium containing various concentrations of leucovorin. Colonies are allowed to grow for a lo-day period prior to staining and counting. Cloning efficiency is determined by comparing the number of colonies formed in MTX-treated cells to those of a control without MTX, but with exposure the corresponding leucovorin concentration.

Animal Study:^{^[2]}

Animal Models
Human colorectal HCT116 xenograft tumors (female NSG mice)
Dosages
80 mg/kg
Administration
i.p.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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