BSJ-03-123

Catalog No.S8815 Batch:S881501

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Technical Data

Formula

C47H56N10O11
Molecular Weight 937.01 CAS No. 2361493-16-3
Solubility (25°C)* In vitro DMSO 100 mg/mL (106.72 mM)
Water ˂1 mg/mL
Ethanol ˂1 mg/mL
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% Corn oil
0.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BSJ-03-123 is a phthalimide-based degrader of cyclin-dependent kinase 6 (CDK6). (PROTAC)
Targets
CDK6 [1]
In vitro

BSJ-03-123 is a phthalimide-based degrader that exploits protein-interface determinants to achieve proteome-wide selectivity for the degradation of cyclin-dependent kinase 6 (CDK6). Pharmacologic CDK6 degradation targets a selective dependency of acute myeloid leukemia cells, and transcriptomics and phosphoproteomics profiling of acute degradation of CDK6 enabled dynamic mapping of its immediate role in coordinating signaling and transcription.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    MV4–11, THP-1, MOLM13, P31/FUJ, MOLT4, NCI-H358, HT29, HEK293T and Hs578T cells

  • Concentrations

    50 nM, 100 nM, 200 nM, 500 nM, 1000 nM

  • Incubation Time

    2 h, 4 h, 24 h

  • Method

    For cell cycle measurement, 106 cells per well are seeded in 24-well plates in triplicates. After 24 h of BSJ-03-123 treatment, cells are harvested by centrifugation at 500g for 5 min and washed in 1 ml cold PBS. Cells are spun down again, resuspended in 200 μl PBS and fixed by addition of 800 μl 70% EtOH and incubation for 20 min at −20°C.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.